Gene Rv2725c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly a putative GTPase, modulating activity of HFLK and HFLC proteins. |
| Product | Probable GTP-binding protein HflX |
| Comments | Rv2725c, (MTCY154.05c), len: 495 aa. Probable hflX (hfl for high frequency of lysogenization), GTP-binding protein ,equivalent to Q9CCC0|ML0997 (alias Q49843|HFLX but longer) possible ATP/GTP-binding protein from Mycobacterium leprae (488 aa), FASTA scores: opt: 2562, E(): 1.1e-133, (84.55% identity in 485 aa overlap). Also highly similar to many e.g. Q9XCC1 from Streptomyces fradiae (425 aa), FASTA scores: opt: 1280, E(): 3.2e-63, (57.7% identity in 423 aa overlap); P73965|HFLX|SLR1521 from Synechocystis sp. strain PCC 6803 (534 aa), FASTA scores: opt: 1028, E(): 2.8e-49, (44.7% identity in 414 aa overlap); P25519|HFLX_ECOLI|B4173 from Escherichia coli strain K12 (426 aa), FASTA scores: opt: 916, E(): 3.4e-43, (40.1% identity in 414 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3037427 | 3038914 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2725c|hflX
MPANSDARPAATCHHRVLAMTYPDPPQTGLSDFTPSLGELALEDRSALRRVAGLSTELADVSEVEYRQLRLERVVLVGVWTEGSAADNRASLAELAALAETAGSQVLEGLIQRRDKPDPSTYIGSGKAAELREVIVATGADTVICDGELSPAQLTALEKAVQVKVIDRTALILDIFAQHATSREGKAQVSLAQMEYMLPRLRGWGESMSRQAGGRAGGSGGGVGLRGPGETKIETDRRRIRERMAKLRRDIRAMKQVRDTQRSRRRHSDVPSIAIVGYTNAGKSSLLNALTGAGVLVQDALFATLEPTTRRAEFGDGRPVVLTDTVGFVRHLPTQLVEAFRSTLEEVVHADLLVHVVDGSDGHPLAQIDAVRQVISEVIADHDGDPPPELLVVNKVDVASDLMLAKLRHGLPGAVFVSARTGDGIDALRRRMAELVVPADTAVDVVIPYDRGDLVARVHADGRIQQAEHKPEGTRIKARVPEALAATLREFAPRA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Ribeiro-Guimarães ML et al. [2007]. Comparative genomics of mycobacterial proteases. Homology
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
