Gene Rv2731
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved alanine and arginine rich protein |
| Comments | Rv2731, (MTCY174.11), len: 450 aa. Conserved ala-, arg-rich protein, highly similar in part to Q49849|B2235_F2_77 hypothetical protein from Mycobacterium leprae (266 aa), FASTA scores: opt: 368, E(): 1e-10, (73.5% identity in 83 aa overlap); and Q9KXN9|SC9C5.35 hypothetical 6.5 KDA protein (fragment) from Streptomyces coelicolor (58 aa), FASTA scores: opt: 214, E(): 0.00065, (51.7% identity in 58 aa overlap). Also similar to Q9L296|SCL2.01 hypothetical 37.4 KDA protein (fragment) from Streptomyces coelicolor (328 aa), FASTA scores: opt: 843, E(): 3.7e-33, (45.95% identity in 296 aa overlap) (but N-terminus shorter); and shows some similarity with other proteins e.g. Q26938 kinetoplast-associated protein (KAP) from Trypanosoma cruzi (1052 aa), FASTA scores: opt: 223, E(): 0.0022, (30.3% identity in 297 aa overlap). Start site chosen by RBS and to avoid overlap, although there are several other possible start sites further upstream. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by proteomics at the Statens Serum Institute (Denmark) (See Rosenkrands et al., 2000). Identified in the cytosol, cell wall, and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the aqueous phase of Triton X-114 extracts of M. tuberculosis H37Rv membranes using 1-DGE and MALDI-TOF-MS (See Sinha et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3043026 | 3044378 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2731|Rv2731
MTADEPRSDDSSGSAPQPAATPVPRPGPRPGPRPVPRPTSYPVGAHPPSDPHRFGRIDDDGTVWLVSASGERIVGSWQAGDPEAAFAHFGRRFDDLSTEIMLMDERLASGTGDARKIKAHAIALAETLPTACVLGDVDALADRLTSIRDRAEVIAAADRSRREEHRAAQTARKEALAAEAEELAANATQWKVAGDRLRAILDEWKTISGVDRKVDDALWKRYSTARDTFNRRRGSHFAELDRERSGVRQSKERLCERAEELSESTDWTATSAEFRKLLADWKAAGRASKDVDDALWRRFKAAQDSFFTARNAATAEKEAELRANADAKEALLAEAERLDTTNHEAARAALRSIAEKWDAIGKVSRERAAELERRLRAVEKKVREAGEADWSDPQARARAEQFRARAEQFEHQAEKAAAAGRTKEADEAKANAEQWRQWAEAAADALTRRP
Bibliography
- Rosenkrands I et al. [2000]. Towards the proteome of Mycobacterium tuberculosis. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Sinha S, Kosalai K, Arora S, Namane A, Sharma P, Gaikwad AN, Brodin P and Cole ST [2005]. Immunogenic membrane-associated proteins of Mycobacterium tuberculosis revealed by proteomics. Proteomics
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
