Gene Rv2746c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in acidic phospholipid biosynthesis. This protein probably catalyzes the committed step to the synthesis of the acidic phospholipids [catalytic activity: CDP-diacylglycerol + glycerol-3-phosphate = CMP + 3-(3-phosphatidyl)-glycerol 1-phosphate]. |
| Product | Probable PGP synthase PgsA3 (CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase) (phosphatidylglycerophosphate synthase) |
| Comments | Rv2746c, (MTV002.11c), len: 209 aa. Probable pgsA3, PGP synthase (see citation below), transmembrane protein, equivalent, but longer 19 aa, to Q49839|O08087|PGSA|ML0979 PGSA from Mycobacterium leprae (193 aa), FASTA scores: opt: 925, E(): 3.7e-53, (77.15% identity in 188 aa overlap). Also highly similar to O86813|PGSA phosphatidylglycerophosphate synthase from Streptomyces coelicolor (263 aa), FASTA scores: opt: 692, E(): 6.6e-38, (57.85% identity in 185 aa overlap) (has its N-terminus longer); and similar to others (generally with N-terminus shorter) e.g. Q99XI0|PGSA|SPY2196 phosphatidylglycerophosphate synthase from Streptococcus pyogenes (180 aa), FASTA scores: opt: 368, E(): 5.4e-17, (39.9% identity in 168 aa overlap); Q9ZE96|PGSA_RICPR|PGSA|RP049 CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase from Rickettsia prowazekii (181 aa), FASTA scores: opt: 343, E(): 2.3e-15, (40.1% identity in 172 aa overlap); P06978|PGSA_ECOLI|PGSA|B1912|Z3000|ECS2650 CDP-diacylglycerol--glycerol-3-phosphate 3- phosphatidyltransferase from Escherichia coli strains K12 and O157:H7 (181 aa), FASTA scores: opt: 322, E(): 5.3e-14, (34.45% identity in 180 aa overlap); etc. Also some similarity to PGSA2|Rv1822|MTCY1A11.21c probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase from Mycobacterium tuberculosis (209 aa), FASTA score: (27.1% identity in 166 aa overlap). Contains PS00379 CDP-alcohol phosphatidyltransferases signature. Belongs to the CDP-alcohol phosphatidyltransferase class-I family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3058602 | 3059231 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2746c|pgsA3
MSRSTRYSVAVSAQPETGQIAGRARIANLANILTLLRLVMVPVFLLALFYGGGHHSAARVVAWAIFATACITDRFDGLLARNYGMATEFGAFVDPIADKTLIGSALIGLSMLGDLPWWVTVLILTRELGVTVLRLAVIRRGVIPASWGGKLKTFVQAVAIGLFVLPLSGPLHVAAVVVMAAAILLTVITGVDYVARALRDIGGIRQTAS
Bibliography
- Jackson M et al. [2000]. Phosphatidylinositol is an essential phospholipid of mycobacteria. Homolog Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
