Gene Rv2747
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in arginine biosynthesis [catalytic activity: L-glutamate + acetyl-CoA <=> N-acetyl-L-glutamate + coenzyme A] |
| Product | Probable L-glutamate alpha-N-acetyltranferase ArgA (alpha-N-acetylglutamate synthase) |
| Comments | Rv2747, (MTV002.12), len: 174 aa. Probable argA, alpha-N-acetylglutamate synthase (See Errey et al., 2005). Contains GNAT (Gcn5-related N-acetyltransferase) domain. See Vetting et al. 2005. Equivalent to O05559|ML0978|MLCB33.08 putative acetyltransferase from Mycobacterium leprae (180 aa), FASTA scores: opt: 997, E(): 1.2e-57, (86.8% identity in 174 aa overlap). Also similar to various transferases e.g. Q9X8N2|SCE94.27c putative acetyltransferase from Streptomyces coelicolor (169 aa), FASTA scores: opt: 656, E(): 1.3e-35, (60.35% identity in 164 aa overlap); C-terminus of Q9K3D6|ARGH(A) argininosuccinase and N-acetylglutamate synthase from Moritella sp. 2693 (629 aa), FASTA scores: opt: 243, E(): 2e-08, (31.95% identity in 144 aa overlap); C-terminus of Q9JW21|ARGA or NMA0580 putative acetylglutamate synthase from Neisseria meningitidis serogroup a (436 aa), FASTA scores: opt: 201, E(): 7.8e-06, (32.75% identity in 119 aa overlap); etc. Also similar to hypothetical proteins e.g. O67372|AQ_1359 hypothetical 21.1 KDA protein from Aquifex aeolicus (181 aa), FASTA scores: opt: 348, E(): 1.2e-15, (42.35% identity in 137 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3059262 | 3059786 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2747|argA
VTERPRDCRPVVRRARTSDVPAIKQLVDTYAGKILLEKNLVTLYEAVQEFWVAEHPDLYGKVVGCGALHVLWSDLGEIRTVAVDPAMTGHGIGHAIVDRLLQVARDLQLQRVFVLTFETEFFARHGFTEIEGTPVTAEVFDEMCRSYDIGVAEFLDLSYVKPNILGNSRMLLVL
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Errey JC et al. [2005]. Functional characterization of a novel ArgA from Mycobacterium tuberculosis. Function Product
- Vetting MW et al. [2005]. Structure and functions of the GNAT superfamily of acetyltransferases. Biochemistry
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
