Gene Rv2748c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in cell division processes |
| Product | Possible cell division transmembrane protein FtsK |
| Comments | Rv2748c, (MTV002.13c), len: 883 aa. Possible ftsK, cell division transmembrane protein, equivalent to O05560|ML0977|FTSK|MLCB33.09c cell division protein from Mycobacterium leprae (886 aa), FASTA scores: opt: 3147, E(): 7.9e-175, (78.1% identity in 885 aa overlap). Also similar to other members of the spoIIIE/ftsK family e.g. O86810|SC7C7.05 FTSK homolog from Streptomyces coelicolor (929 aa), FASTA scores: opt: 2256, E(): 3.8e-123, (49.05% identity in 924 aa overlap); Q9CF25|FTSK cell division protein FTSK from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (763 aa), FASTA scores: opt: 1438, E(): 9.1e-76, (37.7% identity in 751 aa overlap); AAK75005|Q97RE4|SP0878 SPOE family protein from Streptococcus pneumoniae (767 aa), FASTA scores: opt: 1405, E(): 7.5e-74, (48.0% identity in 477 aa overlap); P46889|FTSK_ECOLI|B0890 from Escherichia coli strain K12 (1329 aa), FASTA scores: opt: 759, E(): 0, (44.5% identity in 537 aa overlap) (similarity in C-terminal half); etc. Equivalent to AAK47139 from Mycobacterium tuberculosis strain CDC1551 (968 aa) but shorter 85 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the FTSK/SPOIIIE family. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cytosol and cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3059855 | 3062506 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2748c|ftsK
MLGPPGTPRVGRRDAARSLVTLLRRPWQRGEQIAVTSVADGVDGVIATRLAVMSSKTVARSGTRTSRSKATSRGASRSARSAVPRKRSRPVKGVGRPSRRHHRSLLVSTGLACGRAMRAVWMMAAKGTGGAARSIGRARDIEPGHRRDGIALVLLGLAVVVAASSWFDAARPLGAWVDALLRTFIGSAVVMLPLVAAAVAVVLMRTSPNPDSRPRLILGASLIGLSFLGLCHLWAGSPEAPESRLRAAGFIGFAIGGPLSDGLTAWIAAPLLFIGALFGLLLLAGITIREVPDAMRAMFGTRLLPREYADDFEDFADFDGDDADTVEVARQDFSDGYYDEVPLCSDDGPPAWPSAEVPQDDTATIPEASAGRGSGRRGRRKDTQVLDRIVEGPYTLPSLDLLISGDPPKKRSAANTHMAGAIGEVLTQFKVDAAVTGCTRGPTVTRYEVELGPGVKVEKITALQRNIAYAVATESVRMLAPIPGKSAVGIEVPNTDREMVRLADVLTARETRRDHHPLVIGLGKDIEGDFISANLAKMPHLLVAGSTGSGKSSFVNSMLVSLLTRATPEEVRMILIDPKMVELTPYEGIPHLITPIITQPKKAAAALAWLVDEMEQRYQDMQASRVRHIDDFNDKVRSGAITAPLGSQREYRPYPYVVAIVDELADLMMTAPRDVEDAIVRITQKARAAGIHLVLATQRPSVDVVTGLIKTNVPSRLAFATSSLTDSRVILDQAGAEKLIGMGDGLFLPMGASKPLRLQGAYVSDEEIHAVVTACKEQAEPEYTEGVTTAKPTAERTDVDPDIGDDMDVFLQAVELVVSSQFGSTSMLQRKLRVGFAKAGRLMDLMETRGIVGPSEGSKAREVLVKPDELAGTLAAIRGDGGE
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
