Gene Rv2756c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Implicated in methylation of DNA. Component of type I restriction/modification system. It is possible that the M and S subunits together form a methyltransferase (MTASE) that methylates two adenine residues in complementary strands of bipartite DNA recognition sequence. |
| Product | Possible type I restriction/modification system DNA methylase HsdM (M protein) (DNA methyltransferase) |
| Comments | Rv2756c, (MTV002.21c), len: 540 aa. Possible hsdM, type I restriction/modification system DNA methylase (M protein), highly similar to others e.g. Q9P9X8|XF2742 from Xylella fastidiosa (519 aa), FASTA scores: opt: 1613, E(): 1.9e-96, (52.3% identity in 543 aa overlap); O34139|HSDM from Klebsiella pneumoniae (539 aa), FASTA scores: opt: 1267, E(): 4.4e-74, (45.9% identity in 549 aa overlap); P72418|sty|SBLI|HSDM from Salmonella typhimurium (539 aa), FASTA scores: opt: 1263, E(): 8e-74, (45.7% identity in 549 aa overlap); etc. Possible alternative start site (GTG) overlapping with termination codon of previous ORF 90 bp upstream. Note that the corresponding endonuclease (M protein) does not appear to be present in Mycobacterium tuberculosis. |
| Functional category | Information pathways |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3068461 | 3070083 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2756c|hsdM
MPPRKKQAPQAPSTMKELKDTLWKAADKLRGSLSASQYKDVILGLVFLKYVSDAYDERREAIRAELAAEGMEESQIEDLIDDPEQYQGYGVFVVPVSARWKFLAENTKGKPAVGGEPAKNIGQLIDEAMDAVMKANPTLGGTLPRLYNKDNIDQRRLGELIDLFNSARFSRQGEHRARDLMGEVYEYFLGNFARAEGKRGGEFFTPPSVVKVIVEVLEPSSGRVYDPCCGSGGMFVQTEKFIYEHDGDPKDVSIYGQESIEETWRMAKMNLAIHGIDNKGLGARWSDTFARDQHPDVQMDYVMANLPFNIKDWARNEEDPRWRFGVPPANNANYAWIQHILYKLAPGGRAGVVMANGSMSSNSNGEGDIRAQIVEADLVSCMVALPTQLFRSTGIPVCLWFFAKDKAAGKQGSIDRCGQVLFIDARELGDLVDRAERALTNEEIVRIGDTFHAWRGSKSAAVKGIMYEDVPGFCKSATLAEIKATDYALTPGRYVGTPAVEDDGEPIDEKMARLSKALLEAFDESARLERVVREQLGRLR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
