Gene Rv2842c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv2842c, (MTCY24A1.15), len: 183 aa. Conserved protein, similar to Q9Z5J2|MLCB596.11 hypothetical 13.7 KDA protein from Mycobacterium leprae (122 aa), FASTA scores: opt: 192, E(): 2.1e-12, (50.0% identity in 128 aa overlap) (N-terminus shorter). Also similar in part to several hypothetical proteins e.g. Q9KYR2|SC5H4.27 hypothetical 19.8 KDA protein from Streptomyces coelicolor (177 aa), FASTA scores: opt: 288, E(): 2.1e-12, (37.15% identity in 148 aa overlap); O66619|Y260_AQUAE|AQ_260 hypothetical protein from Aquifex aeolicus (158 aa), FASTA scores: opt: 230, E(): 1.7e-08, (31.35% identity in 153 aa overlap); Q9KU82|VC0641 hypothetical protein from Vibrio cholerae (151 aa), FASTA scores: opt: 198, E(): 2.5e-06, (30.9% identity in 152 aa overlap); etc. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3149425 | 3149976 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2842c|Rv2842c
VTTGLPSQRQVIELLGADFACAGYEIEDVVIDARARPPRIAVIADGDAPLDLDTIAALSRRASALLDGLDGANKIRGRYLLEVSSPGVERPLTSEKHFRRARGRKVELVLSDGSRLTGRVGEMRAGTVALVIREDRGWAVREIPLAEIVKAVVQVEFSPPAPAELELAQSSEMGLARGTEAGA
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
