Gene Rv2861c (map)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Removes the amino-terminal methionine from nascent proteins [catalytic activity: L-methionylpeptide + H(2)O = L-methionine + peptide]. |
| Product | Methionine aminopeptidase MapB (map) (peptidase M) |
| Comments | Rv2861c, (MT2929, MTV003.07c), len: 285 aa. mapB (alternate gene name: map), methionine aminopeptidase, equivalent to Q9CBU7|MAPB|ML1576 methionine aminopeptidase from Mycobacterium leprae (285 aa), FASTA scores: opt: 1729, E(): 1e-99, (89.75% identity in 283 aa overlap). Also highly similar to many e.g. Q9RKR2|MAP3 from Streptomyces coelicolor (285 aa), FASTA scores: opt: 1385, E(): 2e-78, (70.65% identity in 283 aa overlap); Q9SW64|C7A10.320|AT4G37040 from Arabidopsis thaliana (Mouse-ear cress) (305 aa), FASTA scores: opt: 914, E(): 3e-49, (50.35% identity in 286 aa overlap); P07906|AMPM_ECOLI|map|B0168|Z0178|ECS0170 from Escherichia coli strains K12 and O157:H7 (264 aa), FASTA scores: opt: 793, E(): 8.5e-42, (51.0% identity in 245 aa overlap); etc. Belongs to peptidase family M24A; also known as the map family 1. Cofactor: cobalt; binds 2 ions per subunit. Note that this gene has an N-terminal extension present in the human map, but not in the prokaryotic map's. An alternative start, with RBS, will give a protein equivalent to the shorter prokaryotic map's. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in the culture filtrate of M. tuberculosis H37Rv but not the membrane protein fraction or whole cell lysates (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3173160 | 3174017 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2861c|mapB
MPSRTALSPGVLSPTRPVPNWIARPEYVGKPAAQEGSEPWVQTPEVIEKMRVAGRIAAGALAEAGKAVAPGVTTDELDRIAHEYLVDNGAYPSTLGYKGFPKSCCTSLNEVICHGIPDSTVITDGDIVNIDVTAYIGGVHGDTNATFPAGDVADEHRLLVDRTREATMRAINTVKPGRALSVIGRVIESYANRFGYNVVRDFTGHGIGTTFHNGLVVLHYDQPAVETIMQPGMTFTIEPMINLGALDYEIWDDGWTVVTKDRKWTAQFEHTLLVTDTGVEILTCL
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Addlagatta A et al. [2005]. Identification of an SH3-binding motif in a new class of methionine aminopeptidases from Mycobacterium tuberculosis suggests a mode of interaction with the ribosome. Structure
- Ribeiro-Guimarães ML et al. [2007]. Comparative genomics of mycobacterial proteases. Homology
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
