Gene Rv2905
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Probable conserved alanine rich lipoprotein LppW |
| Comments | Rv2905, (MTCY274.36), len: 314 aa. Probable lppW, conserved ala-rich lipoprotein, with slight similarity to beta-lactamases and hypothetical proteins e.g. Q9S1P7|SCJ9A.23 hypothetical 36.3 KDA protein from Streptomyces coelicolor (336 aa), FASTA scores: opt: 222, E(): 2.8e-06, (25.5% identity in 298 aa overlap); O69914|SC3C8.01 putative secreted protein from Streptomyces coelicolor (302 aa), FASTA scores: opt: 201, E(): 5.1e-05, (24.9% identity in 257 aa overlap); P14559|BLAC_STRAL beta-lactamase precursor from Streptomyces albus G (314 aa), FASTA scores: opt: 113, E(): 3.3, (25.2% identity in 278 aa overlap); etc. Has signal peptide and appropriately positioned prokaryotic lipoprotein lipid attachment site: attached to the membrane by a lipid anchor (potential). |
| Functional category | Cell wall and cell processes |
| Proteomics | Predicted surface lipoprotein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3214628 | 3215572 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2905|lppW
MRARPLTLLTALAAVTLVVVAGCEARVEAEAYSAADRISSRPQARPQPQPVELLLRAITPPRAPAASPNVGFGELPTRVRQATDEAAAMGATLSVAVLDRATGQLVSNGNTQIIATASVAKLFIADDLLLAEAEGKVTLSPEDHHALDVMLQSSDDGAAERFWSQDGGNAVVTQVARRYGLRSTAPPSDGRWWNTISSAPDLIRYYDMLLDGSGGLPLDRAAVIIADLAQSTPTGIDGYPQRFGIPDGLYAEPVAVKQGWMCCIGSSWMHLSTGVIGPERRYIMVIESLQPADDATARATITQAVRTMFPNGRI
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
