Gene Rv2914c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in signal transduction (via phosphorylation). Thought to be involved in cell division/differentiation [catalytic activity: ATP + a protein = ADP + a phosphoprotein]. |
| Product | Probable transmembrane serine/threonine-protein kinase I PknI (protein kinase I) (STPK I) (phosphorylase B kinase kinase) (hydroxyalkyl-protein kinase) |
| Comments | Rv2914c, (MTCY338.02c), len: 585 aa. Probable pknI, transmembrane serine/threonine-protein kinase (see citation below), ala-rich protein, highly similar to many in Mycobacterium tuberculosis and other bacteria e.g. Q9RLQ7|MBK putative serine/threonine protein kinase from Mycobacterium bovis BCG (291 aa), FASTA scores: opt: 376, E(): 1.1e-10, (36.95% identity in 287 aa overlap); P33973|PKN1_MYXXA serine/threonine-protein kinase from Myxococcus xanthus (693 aa), FASTA scores: opt: 286, E(): 5.4e-10, (29.9% identity in 374 aa overlap); P72003|PKNF_MYCTU|Rv1746|MT1788|MTCY28.09 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (476 aa), FASTA scores: opt: 675, E(): 1.7e-24, (39.75% identity in 468 aa overlap); Q10697|PKNJ_MYCTU|Rv2088|MT2149|MTCY49.28 probable serine/threonine-protein kinase from Mycobacterium tuberculosis (589 aa), FASTA scores: opt: 574, E(): 1e-19, (34.85% identity in 479 aa overlap); etc. Equivalent to AAK47308 from Mycobacterium tuberculosis strain CDC1551 (603 aa) but shorter 18 aa. Contains Hank's kinase subdomain. Belongs to the Ser/Thr family of protein kinases. |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis H37Rv pknI|Rv2914c mutant shows increased growth in THP-1 cells compared to wild-type; SCID mice infected with wild-type survive longer than those infected with mutant (See Gopalaswamy et al., 2009). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3221767 | 3223524 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2914c|pknI
MALASGVTFAGYTVVRMLGCSAMGEVYLVQHPGFPGWQALKVLSPAMAADDEFRRRFQRETEVAARLFHPHILEVHDRGEFDGQLWIAMDYVDGIDATQHMADRFPAVLPVGEVLAIVTAVAGALDYAHQRGLLHRDVNPANVVLTSQSAGDQRILLADFGIASQPSYPAPELSAGADVDGRADQYALALTAIHLFAGAPPVDRSHTGPLQPPKLSAFRPDLARLDGVLSRALATAPADRFGSCREFADAMNEQAGVAIADQSSGGVDASEVTAAAGEEAYVVDYPAYGWPEAVDCKEPSARAPAPAAPTPQRRGSMLQSAAGVLARRLDNFSTATKAPASPTRRRPRRILVGAVAVLLLAGLFAVGIVIGRKTNTTATEVARPPTSGSAVPSAPTTTVAVTAPVPLDGTYRIEIQRSKQTYDYTPTPQPPDVNTWWAFRTSCTPTECLAAATMLDDNDHTQAKTPPVRPFLMQFGEGQWKSRPETVQFPCVGPNGSPSTQATTQLLALRPQPQGDLVGEMVVTVHSNECGQQGAVIRIPAVASRSGDLPPAVTVPDPATIPDTPDTTSTATLTPPTTTAPGPGR
Bibliography
- Av-Gay Y et al. [2000]. The eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis. Review
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Gopalaswamy R et al. [2009]. The serine/threonine protein kinase PknI controls the growth of Mycobacterium tuberculosis upon infection. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
