Gene Rv2939
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in phthiocerol dimycocerosate (dim) biosynthesis. |
| Product | Possible conserved polyketide synthase associated protein PapA5 |
| Comments | Rv2939, (MTCY19H9.07), len: 422 aa. Possible papA5, conserved polyketide synthase (PKS) associated protein (see Camacho et al., 2001), equivalent to Q49939 hypothetical 45.6 KDA protein from Mycobacterium leprae (423 aa), FASTA scores: opt: 2398, E(): 4.5e-144, (84.05% identity in 426 aa overlap); and Q02279|YMA3_MYCBO hypothetical 38.1 KDA protein from Mycobacterium bovis (354 aa), FASTA scores: opt: 2193, E(): 3.6e-131, (97.4% identity in 343 aa overlap). And C-terminus highly similar to to Q9S381 hypothetical 5.0 KDA protein (fragment) from Mycobacterium leprae (44 aa), FASTA scores: opt: 275, E(): 1.4e-10, (88.65% identity in 44 aa overlap). Also similar in part to various synthetases e.g. Q9AE01|RIF20 RIF20 protein from Amycolatopsis mediterranei (Nocardia mediterranei) (403 aa), FASTA scores: opt: 282, E(): 2.7e-10, (30.3% identity in 393 aa overlap); middle part of Q00869|ESYN1 enniatin sythetase (fragment) (N-methyl peptide synthetase) from Fusarium equiseti (3131 aa), FASTA scores: opt: 180, E(): 0.0036, (26.85% identity in 242 aa overlap); N-terminus of Q9FB18 peptide synthetase NRPS2-1 from Streptomyces verticillus (2626 aa), FASTA scores: opt: 159, E(): 0.068, (23.65% identity in 351 aa overlap); etc. Note that Rv2939|papA5 belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentally). |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 24h of starvation (see Betts et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3274949 | 3276217 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2939|papA5
MFPGSVIRKLSHSEEVFAQYEVFTSMTIQLRGVIDVDALSDAFDALLETHPVLASHLEQSSDGGWNLVADDLLHSGICVIDGTAATNGSPSGNAELRLDQSVSLLHLQLILREGGAELTLYLHHCMADGHHGAVLVDELFSRYTDAVTTGDPGPITPQPTPLSMEAVLAQRGIRKQGLSGAERFMSVMYAYEIPATETPAVLAHPGLPQAVPVTRLWLSKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRFVLAPPVAPTEATNLLGAASYLAEIGPNTDIVDLASDIVATLRADLANGVIQQSGLHFGTAFEGTPPGLPPLVFCTDATSFPTMRTPPGLEIEDIKGQFYCSISVPLDLYSCAVYAGQLIIEHHGHIAEPGKSLEAIRSLLCTVPSEYGWIME
Bibliography
- Camacho LR et al. [2001]. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. Mutant Function
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Buglino J et al. [2004]. Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis. Structure
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
