Gene Rv2943
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Required for the transposition of the insertion element IS1533. |
| Product | Probable transposase for insertion sequence element IS1533 |
| Comments | Rv2943, (MTCY24G1.06c), len: 413 aa. Probable transposase for insertion sequence IS1533, similar to other transposases e.g. P15025|ISTA_ECOLI ista protein (insertion sequence IS21) from Escherichia coli (390 aa), FASTA scores: opt: 268, E(): 5.1e-11, (24.1% identity in 378 aa overlap). Contains potential helix-turn-helix motif at aa 19-40 (Score 1611, +4.67 SD). |
| Functional category | Insertion seqs and phages |
| Proteomics | Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3288464 | 3289705 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2943|Rv2943
MLTVEDWAEIRRLHRAEGLPIKMIARVLGISKNTVKSALESNQQPKYERAPQGSIVDAVEPRIRELLQAYPTMPATVIAERIGWERSIRVLSARVAELRPVYLPPDPASRTTYVAGEIAQCDFWFPPIELPVGFGQTRTAKQLPVLTMVCAYSRWLLAMLLPSRCAEDLFAGWWRLIEALGAVPRVLVWDGEGAIGRWRGGRSELTTECQAFRGTLAAKVLICRPADPEAKGLIERAHDYLERSFLPGRVFASPADFNAQLGAWLALVNTRTRRALGCAPTDRIGADRAAMLSLPPVAPATGWCTSLRLPRDHYVRCDSNDYSVHPGVIGHRVLVRADLERVHVFCDGELVADHERIWAVHQTVSDPAHVEAAKVLRRRHFSAASPVVEPQVQVRSLSDYDDALGVDIDGGVA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
