Gene Rv2950c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in biosynthesis of phenolic glycolipids (PGLs) |
| Product | Fatty-acid-AMP ligase FadD29 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) |
| Comments | Rv2950c, (MTCY349.40), len: 619 aa. fadD29, fatty-acid-AMP synthetase, similar to various mycobacterial enzymes believed to be involved in polyketide or fatty acid synthesis. Equivalent (but shorter 61 aa) to Q9CD84 from Mycobacterium leprae (680 aa), FASTA scores: opt: 3280, E(): 2.2e-192, (80.15% identity in 620 aa overlap); and highly similar to others from Mycobacterium leprae e.g. Q9Z5K5 probable acyl-CoA synthase (583 aa), FASTA scores: opt: 2358, E(): 3.4e-136, (62.35% identity in 579 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. Q10976|FD26_MYCTU putative fatty-acid--CoA ligase (583 aa), FASTA scores: opt: 2416, E(): 1e-139, (63.15% identity in 581 aa overlap) (N-terminus shorter); etc. Equivalent to AAK47349 from Mycobacterium tuberculosis strain CDC1551 (582 aa) but longer 37 aa. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 24h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis H37Rv fadD29|Rv2950c mutant produces phthiocerol and phthiodiolone dimycocerosates (DIMs) but not phenolic glycolipids (PGLs) (See Simeone et al., 2010). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3300596 | 3302455 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2950c|fadD29
MKTNSSFHAAGEVATQPAWGTGEQAAQPLNGSTSRFAMSESSLADLLQKAASQYPNRAAYKFIDYDTDPAGFTETVTWWQVHRRAMIVAEELWIYASSGDRVAILAPQGLEYIIAFMGVLQAGLIAVPLPVPQFGIHDERISSALRDSAPSIILTTSSVIDEVTTYAPHACAAQGQSAPIVVAVDALDLSSSRALDPTRFERPSTAYLQYTSGSTRAPAGVVLSHKNVITNCVQLMSDYIGDSEKVPSTPVSWLPFYHDMGLMLGIILPMINQDTAVLMSPMAFLQRPARWMQLLAKHRAQISSAPNFGFELAVRRTSDDDMAGLDLGHVRTIVTGAERVNVATLRRFTERFAPFNLSETAIRPSYGLAEATVYVATAGPGRAPKSVCFDYQQLSVGQAKRAENGSEGANLVSYGAPRASTVRIVDPETRMENPAGTVGEIWVQGDNVGLGYWRNPQQTEATFRARLVTPSPGTSEGPWLRTGDLGVIFEGELFITGRIKELLVVDGANHYPEDIEATIQEITGGRVVAIAVPDDRTEKLVTIIELMKRGRTDEEEKNRLRTVKREVASAISRSHRLRVADVVMVAPGSIPVTTSGKVRRSASVERYLHHEFSRLDAMA
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Trivedi OA et al. [2004]. Enzymic activation and transfer of fatty acids as acyl-adenylates in mycobacteria. Function Product
- Arora P et al. [2009]. Mechanistic and functional insights into fatty acid activation in Mycobacterium tuberculosis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Siméone R et al. [2010]. Delineation of the roles of FadD22, FadD26 and FadD29 in the biosynthesis of phthiocerol dimycocerosates and related compounds in Mycobacterium tuberculosis. Function Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
