Gene Rv2957
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible glycosyl transferase |
| Comments | Rv2957, (MTCY349.31c), len: 275 aa. Possible glycosyl transferase ; possibly secreted protein. Highly similar to O88109|GSD|GTFD GSD protein from Mycobacterium avium subsp. silvaticum, Mycobacterium paratuberculosis, and Mycobacterium avium (266 aa), FASTA scores: opt: 1010, E(): 2.5e-62, (68.8% identity in 221 aa overlap). Also some similarity with other proteins and especially glycosyl transferases e.g. Q9AEE4 hypothetical 31.4 KDA protein from Leptospira interrogans (265 aa), FASTA scores: opt: 371, E(): 3.3e-18, (34.43% identity in 212 aa overlap); Q9EXY4 putative glycosyl transferase from Escherichia coli (248 aa), FASTA scores: opt: 339, E(): 5e-16, (32.4% identity in 210 aa overlap); Q9RCC4 glycosyltransferase-like protein from Yersinia pestis (247 aa), FASTA scores: opt: 333, E(): 1.3e-15, (31.8% identity in 217 aa overlap); Q9EXY1 putative glycosyl transferase from Escherichia coli (248 aa), FASTA scores: opt: 328, E(): 2.9e-15, (31.9% identity in 210 aa overlap); etc. Equivalent to AAK47357 from Mycobacterium tuberculosis strain CDC1551 (256 aa) but longer 19 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3309470 | 3310297 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2957|Rv2957
MVQTKRYAGLTAANTKKVAMAAPMFSIIIPTLNVAAVLPACLDSIARQTCGDFELVLVDGGSTDETLDIANIFAPNLGERLIIHRDTDQGVYDAMNRGVDLATGTWLLFLGADDSLYEADTLARVAAFIGEHEPSDLVYGDVIMRSTNFRWGGAFDLDRLLFKRNICHQAIFYRRGLFGTIGPYNLRYRVLADWDFNIRCFSNPALVTRYMHVVVASYNEFGGLSNTIVDKEFLKRLPMSTRLGIRLVIVLVRRWPKVISRAMVMRTVISWRRRR
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
