Gene Rv2958c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. Possibly involved in resistance to killing by human macrophages. |
| Product | Possible glycosyl transferase |
| Comments | Rv2958c, (MTCY349.30), len: 428 aa. Possible glycosyl transferase (see citation below), highly similar to Q9CD88|ML0128 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2116, E(): 5.8e-126, (75.05% identity in 417 aa overlap); and Q9CD91|ML0125 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2104, E(): 3.3e-125, (74.65% identity in 418 aa overlap). Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3 putative glycosyltransferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 317, E(): 1.9e-12, (31.0% identity in 297 aa overlap); Q9S1V2 putative glycosyl transferase from Streptomyces coelicolor (407 aa), FASTA scores: opt: 264, E(): 4.1e-09, (27.2% identity in 342 aa overlap); P72650|CRTX|SLR1125 zeaxanthin glucosyl transferase from Synechocystis sp. strain PCC 6803 (419 aa), FASTA scores: opt: 251, E(): 2.8e-08, (26.8% identity in 295 aa overlap); etc. Very similar to P95130|MTCY349.25 from Mycobacterium tuberculosis (449 aa), FASTA score: opt: 2215, E(): 3.3e-132, (77.25% identity in 422 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3310714 | 3312000 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2958c|Rv2958c
MEETSVAGDPGPDAGTSTAPNAAPEPVARRQRILFVGEAATLAHVVRPFVLARSLDPSRYEVHFACDPRFNKLLGPLPFPHHPIHTVPSEEVLLKIAQGRLFYNTRTLRKYIAADRKILNEIAPDVVVGDNRLSLSVSARLAGIPYIAIANAYWSPQARRRFPLPDVPWTRFFGVRPVSILYRLYRPLIFALYCLPLNWLRRKHGLSSLGWDLCRIFTDGDYTLYADVPELVPTYNLPANHRYLGPVLWSPDVKPPTWWHSLPTDRPIIYATLGSSGGKNLLQVVLNALADLPVTVIAATAGRNHLKNVPANAFVADYLPGEAAAARSAVVLCNGGSPTTQQALAAGVPVIGLPSNMDQHLNMEALERAGAGVLLRTERLNTEGVAAAVKQVLSGAEFRQAARRLAEAFGPDFAGFPQHIESALRLVC
Bibliography
- Miller BH et al. [2000]. Evaluation of Mycobacterium tuberculosis genes involved in resistance to killing by human macrophages. Secondary Product
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
