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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionThought to cause methylation.
ProductPossible methyltransferase (methylase)
CommentsRv2959c, (MTCY349.29), len: 245 aa. Possible methyltransferase, highly similar to Q9CD89|ML0127 from Mycobacterium leprae (229 aa), FASTA scores: opt: 1183, E(): 3.9e-69, (76.1% identity in 226 aa overlap). Also some similarity with other methyltransferases and other proteins e.g. Q51079 putative methyl transferase from Nocardia lactamdurans (236 aa), FASTA scores: opt: 156, E(): 0.0086, (23.25% identity in 159 aa overlap); Q98ID5 cephalosporin hydroxylase from Rhizobium loti (Mesorhizobium loti) (217 aa), FASTA scores: opt: 275, E(): 1.7e-10, (29.65% identity in 199 aa overlap); etc. And also similar to P72897 hypothetical 27.8 KDA protein from Mycobacterium tuberculosis (249 aa), FASTA scores: opt: 292, E(): 1.5e-11, (31.25% identity in 208 aa overlap). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate; enriched in the membrane fraction and predicted N-terminal signal peptide is uncleaved (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS33121013312838-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2959c|Rv2959c
MGLVWRSRTSLVGQLIGLVRLVASFAAQLFYRPSDAVAEEYHKWYYGNLVWTKTTYMGINCWKSVSDMWNYQEILSELQPSLVIEFGTRYGGSAVYFANIMRQIGQPFKVLTVDNSHKALDPRARREPDVLFVESSSTDPAIAEQIQRLKNEYPGKIFAILDSDHSMNHVLAEMKLLRPLLSAGDYLVVEDSNINGHPVLPGFGPGPYEAIEAYEDEFPNDYKHDAERENKFGWTSAPNGFLIRN