Gene Rv2962c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. Possibly involved in resistance to killing by human macrophages. |
| Product | Possible glycosyl transferase |
| Comments | Rv2962c, (MTCY349.25), len: 449 aa. Possible glycosyl transferase (see citation below), highly similar or identical to Mycobacterium tuberculosis proteins G560522 U0002JA, G560521 U0002H, G560522 U0002JA, G560519 U0002KA. Equivalent (but longer 21 aa) to Q9CD91 putative glycosyl transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2229, E(): 1.3e-133, (77.45% identity in 426 aa overlap); and highly similar to Q9CD88 putative glycosyl transferase from Mycobacterium leprae (435 aa), FASTA scores: opt: 2129, E(): 2.7e-127, (74.35% identity in 425 aa overlap); and others from Mycobacterium leprae. Also shows some similarity to variety of glycosyl transferases e.g. Q9RYI3|DRA0329 putative glycosyl transferase from Deinococcus radiodurans (418 aa), FASTA scores: opt: 340, E(): 5.5e-14, (31.2% identity in 330 aa overlap); P72650 zeaxanthin glucosyl transferase from Synechocystis sp. (strain PCC 6803) (419 aa), FASTA scores: opt: 244, E(): 6.6e-08, (26.2% identity in 294 aa overlap); etc. Also highly similar to P95134 hypothetical 46.8 KDA protein from Mycobacterium tuberculosis (428 aa), FASTA scores: opt: 2215, E(): 9.6e-133, (77.25% identity in 422 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3313773 | 3315122 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2962c|Rv2962c
VRVSCVYATASRWGGPPVASEVRGDAAISTTPDAAPGLAARRRRILFVAEAVTLAHVVRPFALAQSLDPSRYEVHFACDPRYNQLLGPLPFRHHAIHTIPSERFFGNLTQGRFYAMRTLRKYVEADLRVLDEIAPDLVVGDLRISLSVSARLAGIPYIAIANAYWSPYAQRRFPLPDVIWTRLFGVRLVKLLYRLERPLLFALQCMPLNWVRRRHGLSSLGWNLCRIFTDGDHTLYADVPELMPTYDLPANHEYLGPVLWSPAGKPPTWWDSLPTDRPIVYATLGTSGGRNLLQLVLNALAELPVTVIAATAGRSDLKTVPANAFVADYLPGEAAAARSAVVVCNGGSLTTQQALVAGVPVIGVAGNLDQHLNMEAVERAGAGVLLRTERLKSQRVAGAVMQVISRSEYRQAAARLADAFGRDRVGFPQHVENALRLMPENRPRTWLAS
Bibliography
- Miller BH et al. [2000]. Evaluation of Mycobacterium tuberculosis genes involved in resistance to killing by human macrophages. Secondary Product
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
