Go to browser
virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionCritical role in recombination and DNA repair. Help process holliday junction intermediates to mature products by catalysing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RECG unwind branched duplex DNA (Y-DNA).
ProductProbable ATP-dependent DNA helicase RecG
CommentsRv2973c, (MTCY349.14), len: 737 aa. Probable recG, ATP-dependent DNA helicase (see citation below), equivalent to O69460|RECG_MYCLE ATP-dependent DNA helicase from Mycobacterium leprae (743 aa), FASTA scores: opt: 3846, E(): 0, (79.3% identity in 744 aa overlap). Also highly similar to others e.g. Q9ZBR3|SC7A1.10 putative ATP-dependent DNA helicase from Streptomyces coelicolor (742 aa), FASTA scores: opt: 1249, E(): 1.1e-67, (46.2% identity in 758 aa overlap); Q9PGE8 ATP-dependent DNA helicase from Xylella fastidiosa (718 aa), FASTA scores: opt: 1174, E(): 3.5e-63, (42.1% identity in 539 aa overlap); P24230|RECG_ECOLI|RECG|B3652 from Escherichia coli strain K12 (693 aa), FASTA scores: opt: 457, E(): 7.3e-22, (35.2% identity in 733 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the helicase family, RECG subfamily.
Functional categoryInformation pathways
ProteomicsIdentified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS33277333329946-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2973c|recG
VASLSDRLDRVLGATAADALDEQFGMRTVDDLLRHYPRSYVEGAARVGIGDARPEAGEHITIVDVITDTYSFPMKKKPNRKCLRITVGGGRNKVTATFFNADYIMRDLTKHTKVMLSGEVGYYKGAMQLTHPAFLILDSPDGKNHGTRSLKSIADASKAISGELVVEEFERRFFPIYPASTKVQSWDIFKCVRQVLDVLDRVDDPLPAELRAKHGLIPEDEALRAIHLAESQSLRERARERLTFDEAVGLQWALVARRHGELSESGPSAAWKSNGLAAELLRRLPFELTAGQREVLDVLSDGLAANRPLNRLLQGEVGSGKTIVAVLAMLQMVDAGYQCALLAPTEVLAAQHLRSIRDVLGPLAMGGQLGGAENATRVALLTGSMTAGQKKQVRAEIASGQVGIVIGTHALLQEAVDFHNLGMVVVDEQHRFGVEQRDQLRAKAPAGITPHLLVMTATPIPRTVALTVYGDLETSTLRELPLGRQPIATNVIFVKDKPAWLDRAWRRIIEEAAAGRQAYVVAPRIDESDDTDVQGGVRPSATAEGLFSRLRSAELAELRLALMHGRLSADDKDAAMAAFRAGEVDVLVCTTVIEVGVDVPNATVMLVMDADRFGISQLHQLRGRIGRGEHPSVCLLASWVPPDTPAGQRLRAVAGTMDGFALADLDLKERKEGDVLGRNQSGKAITLRLLSLAEHEEYIVAARDFCIEAYKNPTDPALALMAARFTSTDRIEYLDKS