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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in thiamine biosynthesis [catalytic activity: ATP + thiamine phosphate = ADP + thiamine diphosphate].
ProductProbable thiamine-monophosphate kinase ThiL (thiamine-phosphate kinase)
CommentsRv2977c, (MTCY349.10), len: 333 aa. Possible thiL, thiamin-monophosphate kinase, equivalent to Q9CBS2 probable thiamine-monophosphate kinase from Mycobacterium leprae (325 aa), FASTA scores: opt: 1738, E(): 4.5e-98, (80.9% identity in 314 aa overlap). Also highly similar to others e.g. Q9ZBR7|SC7A1.06 putative thiamine monphosphate kinase from Streptomyces coelicolor (322 aa), FASTA scores: opt: 959, E(): 7.8e-51, (51.1% identity in 319 aa overlap); O05514|THIL_BACSU thiamine-monophosphate kinase from Bacillus subtilis (325 aa), FASTA scores: opt: 476, E(): 1.5e-21, (35.15% identity in 273 aa overlap); P77785|THIL_ECOLI|THIL|B0417 thiamine-monophosphate kinase from Escherichia coli strain K12 (325 aa), FASTA scores: opt: 418, E(): 5e-18, (36.9% identity in 282 aa overlap); etc. Belongs to the thiamine-monophosphate kinase family. Note that the start, as given, is in IS1538.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS33327873333788-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2977c|thiL
VTTKDHSLATESPTLQQLGEFAVIDRLVRGRRQPATVLLGPGDDAALVSAGDGRTVVSTDMLVQDSHFRLDWSTPQDVGRKAIAQNAADIEAMGARATAFVVGFGAPAETPAAQASALVDGMWEEAGRIGAGIVGGDLVSCRQWVVSVTAIGDLDGRAPVLRSGAKAGSVLAVVGELGRSAAGYALWCNGIEDFAELRRRHLVPQPPYGHGAAAAAVGAQAMIDVSDGLLADLRHIAEASGVRIDLSAAALAADRDALTAAATALGTDPWPWVLSGGEDHALVACFVGPVPAGWRTIGRVLDGPARVLVDGEEWTGYAGWQSFGEPDNQGSLG