Gene Rv2978c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Required for the transposition of the insertion element IS1538. |
| Product | Probable transposase |
| Comments | Rv2978c, (MTCY349.09), len: 459 aa. Probable transposase for IS1538, very similar to several other putative transposases from Mycobacterium tuberculosis e.g. YX16_MYCTU|Q10809 (460 aa), FASTA scores: opt: 2613, E(): 0, (83.0% identity in 458 aa overlap); etc. Low level matches to other tranposases. |
| Functional category | Insertion seqs and phages |
| Proteomics | Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3333785 | 3335164 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2978c|Rv2978c
MPKFEVPDGWTVQAFRFTLDPTEDQAKALARHFGARRKAYNWTVATLKADIQAWHASGTVTAKPSLRVLRKRWNTVKDDVCVNTETGVAWWPECSKEAYADGIAGAVEAYWNWQTSRAGKRAGKRVGFPRFKRKGRDQDRVSFTTGAMRVEPDRRHLTLPVIGTVRTHENTRRIERLIKAGRARVLAISVRRNGTRLDASVRVLVQRPQQPKVVHPGSRVGVDVGVRRLATVATADGTAIEQVENPRPLGAALRELRHVCRARSRCTKGSRRYRERTTQISRLHRRVNDVRTHHLHVLTTRLAQTHGRIVVEGLDATEMLRQKGLPGARARRRGLSDAALGTPRRHLSYKTVWYGSALVVADRWFPSSKTCHACRHVQDIGWDEQWQCDRCSVVHQRDDCAAINLARYEETSSIVGPVGAAVKRGADRKTGPRPAGGCEARKGSSPKAAEQPRDGVQVA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
