Gene Rv2997
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible alanine rich dehydrogenase |
| Comments | Rv2997, (MTV012.11), len: 480 aa. Possible ala-rich dehydrogenase, similar to others dehydrogenases and hypothetical proteins e.g. Q9EYI5 putative dehydrogenase from Streptomyces nogalater (472 aa), FASTA scores: opt: 1131, E(): 1.7e-61, (41.0% identity in 471 aa overlap); Q9ZBG4|SC9B5.16 putative dehydrogenase from Streptomyces coelicolor (472 aa), FASTA scores: opt: 1064, E(): 2e-57, (39.05% identity in 471 aa overlap); Q98BS8 probable dehydrogenase from Rhizobium loti (Mesorhizobium loti) (524 aa), FASTA scores: opt: 196, E(): 0.00021, (25.1% identity in 526 aa overlap); etc. Shows strong similarity throughout its length to O06826|MTCY493.22c|Rv1432 hypothetical 50.5 KDA protein from Mycobacterium tuberculosis (473 aa), FASTA scores: opt: 1220, E(): 6.1e-67, (42.35% identity in 465 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3355099 | 3356541 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2997|Rv2997
VDVTVVGSGPNGLATAVICARAGLNVQVVEAQATFGGGARSAADFEFPEVLHDVCSAVHPLALASPFFAEFDLPARGVTLTVPDIAYANPLPGRPAAIAYHDLAHTCAKLDDGASWRRLLGPLVAHSETVVEFMLSDKRSLPTALGSVLRLGLRMLAQGTPAWRSLAGEDARALFTGVAAHAISPLPSLVSAGAGLMLATLAHSVGWPIPVGGTQAIADALIADLRAHGGRLAAGVEITEPQRSVVVFDTAPTALLRVYRDKLPHRYAKALRRYRFRAGIAKVDFVLSDEIPWSDPRLRRAATLHLGGTRDQMARAEADVAAGRHADWPMVLAACPHVADPGRIDETGRRPFWTYAHVPSGSTLDATETVTSVLERFAPGFRDIVVAARAVPAARMADHNANYVGGDITVGANSTWRAIAGPTPRLNPWRTPIPKVYLCSAATPPGAGVHGMCGWYAARTLLRTEFGITRMPPLGHELRP
Bibliography
- Ehrt S et al. [1997]. A novel antioxidant gene from Mycobacterium tuberculosis. Sequence
- Stewart GR et al. [2000]. Deletion of the putative antioxidant noxR1 does not alter the virulence of Mycobacterium tuberculosis H37Rv. Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
