Gene Rv2999
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Probable conserved lipoprotein LppY |
| Comments | Rv2999, (MTV012.13), len: 321 aa. Probable lppY, conserved lipoprotein, highly similar to O07774|LPQO|Rv0604|MTCY19H5.18c putative lipoprotein from Mycobacterium tuberculosis (316 aa), FASTA scores: opt: 1153, E(): 5e-62, (53.2% identity in 312 aa overlap); and showing similarity with AAK80743|CAC2799 uncharacterized conserved protein similar to LPPY/LPQO of Mycobacterium tuberculosis from Clostridium acetobutylicum (152 aa), FASTA scores: opt: 165, E(): 0.0077, (26.08% identity in 138 aa overlap); and Q9F2T1|SCD65.01c putative lipoprotein (fragment) from Streptomyces coelicolor (146 aa), FASTA scores: opt: 126, E(): 1.6, (% identity in aa overlap). Equivalent to AAK47407 from Mycobacterium tuberculosis strain CDC1551 (329 aa) but shorter 8 aa. Contains probable N-terminal signal sequence and PS00013 Prokaryotic membrane lipoprotein lipid attachment site. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3357602 | 3358567 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2999|lppY
MAGAKHAGRIVAITTAAAVILAACSSGSKGGAGSGHAGKARSAVTTTDADWKPVADALGRSGKLGDNNTAYRINLPRNDLHITSYGVDIKPGLSLGGYAAFARYDNNETLLMGDLVITEEELPKVTDALQAHGIAQTALHKHLLQQDPPVWWTHIHGMGDAARLAQGLKAALDATTIGPPTPPPARQPPVDIDVAGVDQALGRKGTQDGGLMKYSIPRKDTIIEDGHVLPAVSLNLTTVINFQPVGRGRAAINGDFILIAPEVQEVIRAMRAGNITIVELHNHGLTEEPRLFYMHYWAVDDAVTLARALRPAMDATNLQSS
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
