Gene Rv3010c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in glycolysis; converts sugar-1-P to sugar-1,6-P [catalytic activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate]. |
| Product | Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase) |
| Comments | Rv3010c, (MTV012.24c), len: 343 aa. Probable pfkA, phosphofructokinase, equivalent to O33106|K6PF_MYCLE|MLCB637.14 6-phosphofructokinase from Mycobacterium leprae (343 aa), FASTA scores: opt: 2099, E(): 4.1e-122, (90.4% identity in 343 aa overlap). Also highly similar to others e.g. Q9FC99|K6P3_STRCO from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1329, E(): 1.1e-74, (58.9% identity in 338 aa overlap); Q9L1L8|K6P2_STRCO|PFKA2|PFK2|SC6A11.02 6-phosphofructokinase 2 from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1303, E(): 4.5e-73, (56.7% identity in 342 aa overlap); Q9KH71|PFP PPI-dependent phosphofructokinase from Dictyoglomus thermophilum (346 aa), FASTA scores: opt: 893, E(): 8.4e-48, (41.85% identity in 344 aa overlap); etc. Contains PS00433 Phosphofructokinase signature. Belongs to the phosphofructokinase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell wall and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3368823 | 3369854 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3010c|pfkA
MRIGVLTGGGDCPGLNAVIRAVVRTCHARYGSSVVGFQNGFRGLLENRRVQLHNDDRNDRLLAKGGTMLGTARVHPDKLRAGLPQIMQTLDDNGIDVLIPIGGEGTLTAASWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAESHERVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDIEEVCRLVKGRFQRGDSHFICVVAEGAKPAPGTIMLREGGLDEFGHERFTGVAAQLAVEVEKRINKDVRVTVLGHIQRGGTPTAYDRVLATRFGVNAADAAHAGEYGQMVTLRGQDIGRVPLADAVRKLKLVPQSRYDDAAAFFG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
