Gene Rv3060c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Probable transcriptional regulatory protein (probably GntR-family) |
| Comments | Rv3060c, (MTCY22D7.21), len: 490 aa. Probable transcriptional regulatory protein, showing reasonable similarity to several members of the GntR family e.g. BAB54431|MLL8575 from Rhizobium loti (Mesorhizobium loti) (247 aa), FASTA scores: opt: 274, E(): 3.5e-10, (30.35% identity in 224 aa overlap); P96570|ESMR from Burkholderia cepacia (Pseudomonas cepacia) (277 aa), FASTA scores: opt: 229, E(): 2.8e-07, (25.85% identity in 240 aa overlap); Q9S276|SCI28.07 from Streptomyces coelicolor (230 aa), FASTA scores: opt: 211, E(): 3.4e-06, (27.25% identity in 220 aa overlap); etc. Seems to have two domains: residues 1-260 resemble UxuR, and 260-490 resemble PdhR, ExuR, etc. Contains bacterial regulatory proteins, GntR family signature (PS00043). Helix-turn-helix motif (+3.13 SD) at aa 38-59. Seems to belong to the GntR family of transcriptional regulators. |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cell wall and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | mRNA identified by DNA microarray analysis and up-regulated at high temperatures (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3421741 | 3423213 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3060c|Rv3060c
MSTEPDAVWTDKRASKIARRIEADIVRRGWPIGASLGSESALQQRFCVSRSVLREAVRLVEHHQVARMRRGPNGGLFICEPNAGPATRAVVIYLEYLGTTIGDLLGARLVLEPLAASLAAEHIDEPGIERLRAVLRAEERWRPGLPPPPEQFYRVLAEQSKNPVLQLFIDILMRLTKRYVQKSGTQSAGEAVEAAGQVHNEHSDIVAAVTAGDSAWAKTLSERHVEAVAGWLQQHQRGNDAAVRNGGRAREPRRAQQLILGAPRGKLAEVLAATIGDDIAASGWQVGSVFGTETALLERYQVSRAVLREAVRLLEYHAIAHMRRGPGGGLVVTTPQPQASIDTIALYLQYRKPSREDLRCVRDAIEIDNVAKVVKRRSEPEVASFLDTLGRPRLDNPTDDVRAAAVEEFRFHVGLARAAGNTMLDLFLLILVELFRRHLSSTEQALPTWSDVVAVGHAHVRILEAIGSGDDSLARCRTRRHLDAAASWWL
Bibliography
- Stewart GR et al. [2002]. Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays. Transcriptome Mutant Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
