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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionThis protein seals during DNA replication, DNA recombination and DNA repair NICKS in double-stranded DNA [catalytic activity: ATP + (deoxyribonucleotide)(N) + (deoxyribonucleotide)(M) = AMP + pyrophosphate + (deoxyribonucleotide)(N+M)].
ProductProbable ATP-dependent DNA ligase LigB (polydeoxyribonucleotide synthase [ATP]) (polynucleotide ligase [ATP]) (sealase) (DNA repair protein) (DNA joinase)
CommentsRv3062, (MTCY22D7.19c), len: 507 aa. Probable ligB, DNA ligase ATP-dependent (see citation below), highly similar to numerous archaebacterial and eukaryotic polynucleotide DNA ligases, e.g. Q9FCB1|DNLI_STRCO|LIG|2SCG58.02 from Streptomyces coelicolor (512 aa), FASTA scores: opt: 1677, E(): 2.5e-90, (55.65% identity in 512 aa overlap); Q9HR35|DNLI_HALN1|LIG|VNG0881G from Halobacterium sp. strain NRC-1 (561 aa), FASTA scores: opt: 985, E(): 5.6e-50, (42.25% identity in 440 aa overlap); Q9V185|DNLI_PYRAB|LIG|PAB2002 from Pyrococcus abyssi (559 aa), FASTA scores: opt: 978, E(): 1.4e-49, (39.05% identity in 443 aa overlap); etc. Also similar to Rv3731|MTV025.079|LIGC possible DNA ligase from M. tuberculosis (358 aa). Similarity at N-terminus is poor so first start codon was taken. Contains (PS00697) ATP-dependent DNA ligase AMP-binding site signature, and (PS00017) ATP/GTP-binding site motif A (P-loop). Belongs to the ATP-dependent DNA ligase family.
Functional categoryInformation pathways
ProteomicsIdentified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Non essential gene in M. tuberculosis Erdman (See Gong et al., 2004).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS34255843427107+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3062|ligB
VLLHDVAITSMDVAATSSRLTKVARIAALLHRAAPDTQLVTIIVSWLSGELPQRHIGVGWAALRSLPPPAPQPALTVTGVDATLSKIGTLPGKGSQAQRAALVAELFSAATEAEQTFLLRLLGGELRQGAKGGIMADAVAQAAGLPAATVQRAAMLGGDLAAAAAAGLSGAALDTFTLRVGRPIGPMLAQTATSVHDALERHGGTTIFEAKLDGARVQIHRANDQVRIYTRSLDDVTARLPEVVEATLALPVRDLVADGEAIALCPDNRPQRFQVTASRFGRSVDVAAARATQPLSVFFFDILHRDGTDLLEAPTTERLAALDALVPARHRVDRLITSDPTDAANFLDATLAAGHEGVMAKAPAARYLAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHLGARDPATGGFVMVGKTFKGMTDAMLDWQTTRFHEIAVGPTDGYVVQLRPEQVVEVALDGVQRSSRYPGGLALRFARVVRYRADKDPAEADTIDAVRALY