Gene Rv3074
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3074, (MTCY22D7.07c), len: 424 aa. Conserved hypothetical protein, highly similar but shorter (46 aa) to P71806|Rv1378c|MTCY02B12.12c hypothetical 51.3 KDA protein from Mycobacterium tuberculosis (475 aa), FASTA scores: opt: 2009, E(): 5.8e-113, (72.95% identity in 429 aa overlap); and also similar to other hypothetical mycobacterium proteins e.g. O33266|Rv0336|MTCY279.03 (503 aa), FASTA scores: opt: 337, E(): 7.5e-13, (28.6% identity in 381 aa overlap); O33360|Rv0515|MTCY20G10.05 (503 aa), FASTA scores: opt: 337, E(): 7.5e-13, (28.6% identity in 381 aa overlap); etc. |
| Functional category | Conserved hypotheticals |
| Transcriptomics | mRNA identified by Microarray analysis (see Davis et al., 2002). DNA microarrays detect expression in M. tuberculosis H37Rv in vivo (in BALB/c and SCID mice) but not in vitro (in 7H9 medium) (See Talaat et al., 2004). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3436779 | 3438053 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3074|Rv3074
MFETLTAIDPDAEEAALIERIAELERLKSAAAAGQARAAAAVDAARRAAEGAAGVPAARRGRGLASEIALARRDSPARGSRHLGFAKALVYEMPHTLAALDCGALSEWRATLIVRESACLDVADRRALDAELCGDPGDLEGMGDARVVAAARAIAYRLDPQAVVDRAANAENDRTVTIRPAPDTMTYLTALLPVAQGVSVYAALTRAADTRCDGRSRGQVMADTLVERVTGRDAAVPTPIAVNLVMSDETLLGAANTPAQLCGYGPIPAAVARTMVASAVTDQRSRATLRRLYAHPQAGALVSMESRARLFPRGLAAFIELRDQRCRTPYCDAPIRHRDHAHPWADGGPTSAHNGLGTCERCNYAKQAPGWRVSTSVDENHTHTAEFITPTGSRHRSGAPPHLPAVTVSELEVRIGIALARYAA
Bibliography
- Davis EO et al. [2002]. Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis. Sequence Regulation Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
