Gene Rv3089
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid degradation. |
| Product | Probable chain -fatty-acid-CoA ligase FadD13 (fatty-acyl-CoA synthetase) |
| Comments | Rv3089, (MTV013.10), len: 503 aa. Probable fadD13, Acyl-CoA Synthetase, similar to many e.g. MTCI28.06, MTCY08D5.09, MTCY06G11.08 from Mycobacterium tuberculosis strain H37Rv; and to Q9F7P5 predicted acid--CoA ligase FADD13 from uncultured proteobacterium EBAC31A08 (504 aa), FASTA scores: opt: 1126, E(): 2.4e-62, (38.85% identity in 502 aa overlap); Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. strain HR167 (491 aa), FASTA scores: opt: 1073, E(): 4.5e-59, (38.5% identity in 504 aa overlap); BAB49118|MLR1843 probable acid-CoA ligase from Rhizobium loti (Mesorhizobium loti) (495 aa), FASTA scores: opt: 937, E(): 1.2e-50, (36.2% identity in 503 aa overlap); Q9KZC1|SC6F7.21 probable long-chain-fatty-acid-CoA ligase from Streptomyces coelicolor (511 aa), FASTA scores: opt: 899, E(): 2.8e-48, (36.1% identity in 510 aa overlap); Q9A5P7|CC2400 putative acid-CoA ligase from Caulobacter crescentus (496 aa), FASTA scores: opt: 874, E(): 9.8e-47, (35.1% identity in 507 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature and PS00061 Short-chain alcohol dehydrogenase family signature. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in the culture filtrate and whole cell lysates of M. tuberculosis H37Rv but not the membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and real-time RT-PCR; transcription up-regulated at low pH in vitro conditions, which may mimic an environmental signal encountered by phagocytosed bacteria (see Fisher et al., 2002). And mRNA identified by microarray analysis and up-regulated after 4h and 96h of starvation (see Betts et al., 2002). |
| Operon | Rv3088 and Rv3089 are co-transcribed, by RT-PCR (See Singh et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3455761 | 3457272 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3089|fadD13
MKNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYGAPSAPVIDAIRAQADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMRGVTLISMPQFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMPEALIKIYAAKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHGEGEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQNEVSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGKILKTVLREQYSATVPK
Bibliography
- Fisher MA, Plikaytis BB and Shinnick TM [2002]. Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes. Transcriptome Regulation
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Singh R et al. [2005]. Deciphering the genes involved in pathogenesis of Mycobacterium tuberculosis. Operon
- Kumar P et al. [2009]. The Mycobacterium tuberculosis protein kinase K modulates activation of transcription from the promoter of mycobacterial monooxygenase operon through phosphorylation of the transcriptional regulator VirS. Biochemistry
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
