Gene Rv3107c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in ether lipid biosynthesis [catalytic activity: 1-acyl-glycerone 3-phosphate + a long-chain alcohol = 1-alkyl-glycerone 3-phosphate + a long-chain acid anion]. |
| Product | Possible alkyldihydroxyacetonephosphate synthase AgpS (alkyl-DHAP synthase) (alkylglycerone-phosphate synthase) |
| Comments | Rv3107c, (MTCY164.17c), len: 527 aa. Possible agpS, alkyl-dihydroxyacetonephosphate synthase, similar to others and some various enzymes e.g. AAK46595|MT2311 putative alkyl-dihydroxyacetonephosphate synthase from Mycobacterium tuberculosis strain CDC1551 (529 aa), FASTA scores: opt: 1052, E(): 2.1e-58, (37.1% identity in 542 aa overlap); Q9RJ97|SCF91.28c putative flavoprotein from Streptomyces coelicolor (530 aa), FASTA scores: opt: 972, E(): 2.2e-53, (36.2% identity in 544 aa overlap); O96759|ADAS_DICDI alkyldihydroxyacetonephosphate synthase from Dictyostelium discoideum (Slime mold) (611 aa), FASTA scores: opt: 617, E(): 4.5e-31, (33.95% identity in 480 aa overlap); O97157|ADAS_TRYBB alkyldihydroxyacetonephosphate synthase from Trypanosoma brucei (613 aa), FASTA scores: opt: 567, E(): 6.2e-28, (29.15% identity in 521 aa overlap); etc. Also similar to O53525|Rv2251|MTV022.01 hypothetical 49.8 KDA protein from Mycobacterium tuberculosis strain H37Rv (475 aa), FASTA scores: opt: 1019, E(): 2.3e-56, (38.6% identity in 487 aa overlap). Belongs to the FAD-binding oxidoreductase/transferase family 4. Cofactor: FAD (by similarity). |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3475378 | 3476961 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3107c|agpS
MRSWWGWGTVEDALSDQETQALQSRVAALVSGHDLSDHPPPDLTALGLAAPRVSPPASLAALCSSDLVDRAGHARGKAYRDIARNLQGQLDHLPDLIARPRSEQDVIDVLDWCAREGIAVIPYGGGSSVVGGVEPRFDEPVVTVDVTAMSAVLEIDRVSRAARIQAGAFGPSIEHQLRPHDLTLRHFPQSFGFSTLGGWLATRSGGHFATLYTHIDDLTESLRIVTPVGISESRRLPGSGAGPSPDRLFLGSEGTLGIITEAWMRLQHRPRWQVTVSVVFDDWAAAVAATRTIAQAGLYPANCRLLDPAEALLNAGTSVGGGLLVLAFESADHPIDPWLHRAVAITAEHGGTVTAQRSRGTTSDATEHNAAANWRSAFLRMPYQRDALVRRGVIAETFETACTWDGFDTLHAAVTDAARTAIWKVCGTGVVTCRFTHVYPDGPAPYYGIYAGGRWGSLDAQWDEIKAAVSEAISASGGTITHHHAVGRDHRAWYDRQRPDPFAAALRAAKSALDPAGILNPGVLLGR
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
