Gene Rv3114
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3114, (MTCY164.24), len: 176 aa. Conserved hypothetical protein, with some similarity to Q9F9W7 cytosine deaminase from Bifidobacterium longum (143 aa), FASTA scores: opt: 207, E(): 2.2e-07, (37.05% identity in 108 aa overlap); and Q9RV23|DR1207 cell cycle protein MESJ, putative/cytosine deaminase-related protein from Deinococcus radiodurans (600 aa), FASTA scores: opt: 212, E(): 3.5e-07, (33.35% identity in 177 aa overlap). Equivalent to AAK47536|MT3196 cytidine and deoxycytidylate deaminase family protein from Mycobacterium tuberculosis strain CDC1551 (187 aa) but shorter 11 aa. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3480759 | 3481289 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3114|Rv3114
VVAARLPFGWSADSGVTADIIEAAMELAIDTARHATAPFGAALLDVTTLRAFSGGNTYFESGDRFAHAETNVLRAAMSTLPELSNHVLISTAEPCPMCAAASVLSGVRAIIFGTSIETLIQCGWFQIRISASDVVAASTRPTRPSVYSGFLSHKTDLLYRNSENRRAMNPWTDPSH
Bibliography
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
