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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in aerobic|anaerobic respiration [catalytic activity: NADH + ubiquinone = NAD(+) + ubiquinol].
ProductProbable NADH dehydrogenase I (chain C) NuoC (NADH-ubiquinone oxidoreductase chain C)
CommentsRv3147, (MTCY03A2.11c), len: 236 aa. Probable nuoC, NADH dehydrogenase, chain C, similar to others e.g. Q9XAQ6|NUOC from Streptomyces coelicolor (251 aa), FASTA scores: opt: 1113, E(): 2.6e-64, (67.35% identity in 236 aa overlap); Q9A6X2|CC1954 from Caulobacter crescentus (197 aa), FASTA scores: opt: 351, E(): 1.6e-15, (41.65% identity in 132 aa overlap); BAB48757|MLL1369 from Rhizobium loti (Mesorhizobium loti) (201 aa), FASTA scores: opt: 347, E(): 3e-15, (42.4% identity in 132 aa overlap); etc. Also similar to Q9UUU0|NUGM NUGM protein precursor from Yarrowia lipolytica (Candida lipolytica) (281 aa), FASTA scores: opt: 356, E(): 1.1e-15, (34.55% identity in 162 aa overlap). Also similar to MTCY251.05, FASTA score: E():4.9e-05. Equivalent to AAK47574 from Mycobacterium tuberculosis strain CDC1551 but longer 26 aa. Belongs to the complex I 30 KDA subunit family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by proteomics at the Max Planck Institute for Infection Biology, Berlin, Germany (see Jungblut et al., 1999). Identified in the culture supernatant of M. tuberculosis H37Rv using mass spectrometry (See Mattow et al., 2003). Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
TranscriptomicsmRNA identified by microarray analysis and down-regulated after 24h and 96h of starvation (see Betts et al., 2002).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS35126283513338+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3147|nuoC
MSPPNQDAQEGRPDSPTAEVVDVRRGMFGVSGTGDTSGYGRLVRQVVLPGSSPRPYGGYFDDIVDRLAEALRHERVEFEDAVEKVVVYRDELTLHVRRDLLPRVAQRLRDEPELRFELCLGVSGVHYPHETGRELHAVYPLQSITHNRRLRLEVSAPDSDPHIPSLFAIYPTNDWHERETYDFFGIIFDGHPALTRIEMPDDWQGHPQRKDYPLGGIPVEYKGAQIPPPDERRGYN