Gene Rv3163c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Possible conserved secreted protein |
| Comments | Rv3163c, (MTV014.07c), len: 423 aa. Possible conserved secreted protein, with some similarity to other hypothetical bacterial proteins e.g. Q9Z539|SC9B2.20c from Streptomyces coelicolor (460 aa), FASTA scores: opt: 666, E(): 1.5e-33, (33.55% identity in 417 aa overlap); O58486|PH0774 from Pyrococcus horikoshii (410 aa), FASTA scores: opt: 329, E(): 6.9e-13, (23.8% identity in 424 aa overlap); Q9UZ66|PAB0849 from Pyrococcus abyssi (410 aa), FASTA scores: opt: 322, E(): 1.9e-12, (24.15% identity in 389 aa overlap); etc. Also some similarity with P71761|Rv1480|MTV007.27|MTCY277.01 from Mycobacterium tuberculosis (317 aa), FASTA scores: opt: 198, E(): 6.3e-05, (26.75% identity in 269 aa overlap). Contains PS00402 Binding-protein-dependent transport systems inner membrane comp signature. |
| Functional category | Cell wall and cell processes |
| Transcriptomics | mRNA identified by microarray analysis; transcription repressed at low pH in vitro conditions, which may mimic an environmental signal encountered by phagocytosed bacteria (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3531642 | 3532913 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3163c|Rv3163c
VIQTCEVELRWRASQLTLAIATCAGVALAAAVVAGRWQLIAFAAPLLGVLCSISWQRPVPVIQVHGDPDSQRCFENEHVRVTVWVTTESVDAAVELTVSALAGMQFEALESVSRRTTTVSAVAQRWGRYPIRARVAVVARGGLLMGAGTVDAAEIVVFPLTPPQSTPLPQTELLDRLGAHLTRHVGPGVEYADIRPYVPGDQLRAVNWVVSARRGRLHVTRRLTDRAADVVVLIDMYRQPAGPATEATERVVRGAAQVVQTALRNGDRAGIVALGGNRPRWLGADIGQRQFYRVLDTVLGAGEGFENTTGTLAPRAAVPAGAVVIAFSTLLDTEFALALIDLRKRGHVVVAVDVLDSCPLQDQLDPLVVRMWALQRSAMYRDMATIGVDVLSWPADHSLQQSMGALPNRRRRGRGRASRARLP
Bibliography
- Fisher MA, Plikaytis BB and Shinnick TM [2002]. Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes. Transcriptome Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
