Gene Rv3164c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism; regulates methanol dehydrogenase. |
| Product | Probable methanol dehydrogenase transcriptional regulatory protein MoxR3 |
| Comments | Rv3164c, (MTV014.08c), len: 320 aa. Probable moxR3, methanol dehydrogenase regulatory protein, highly similar to Q9Z538|SC9B2.21c putative regulatory protein from Streptomyces coelicolor (332 aa), FASTA scores: opt: 1227, E(): 1.7e-67, (60.25% identity in 302 aa overlap); Q9UZ67|MOXR-3|PAB0848 methanol dehydrogenase regulatory protein from Pyrococcus abyssi (314 aa), FASTA scores: opt: 1126, E(): 2.3e-61, (54.1% identity in 305 aa overlap); Q9HSH7|MOXR|VNG0223G methanol dehydrogenase regulatory protein from Halobacterium sp. strain NRC-1 (318 aa), FASTA scores: opt: 1072, E(): 4.5e-58, (51.45% identity in 315 aa overlap); Q9RVV4|DR0918 MOXR-related protein from Deinococcus radiodurans (354 aa), FASTA scores: opt: 1000, E(): 1.2e-53, (50.95% identity in 318 aa overlap); etc. Also high similarity with several hypothetical bacterial proteins. |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3532943 | 3533905 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3164c|moxR3
MIMPAATTTAHCEAVLDEIERVVVGKRSALTLILTAVLARGHVLIEDLPGLGKTLIARSFAAALGLDFTRVQFTPDLLPADLLGSTIYDMQSGRFEFRAGPIFTNLLLADEINRTPPKTQAALLEAMAEGQVSIDGQTHKLAMPFIVLATDNPIEYEGTYPLPEAQLDRFAIRLELRYLSERDETSMLRRRLERGSADPTVNQVVDCHDLLAMRESVEQVTVHEDVLHYVVSLANATRHHPQVAVGASPRAELDLVQLSRARALLLGRDYVIPEDVKELATAAVAHRITLRPEMWVRKIAGADVVSELLRRLPVPRISGT
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
