Gene Rv3173c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in transcriptional mechanism (probably repression). |
| Product | Probable transcriptional regulatory protein (probably TetR/AcrR-family) |
| Comments | Rv3173c, (MTV014.17c), len: 200 aa. Probable transcriptional regulatory protein TetR family, similar to several bacterial putative regulatory proteins e.g. Q9EWI2|SC7H9.14 from Streptomyces coelicolor (195 aa), FASTA scores: opt: 319, E(): 1.7e-13, (34.55% identity in 195 aa overlap); O85695|3SCF60.04 from Streptomyces lividans and Streptomyces coelicolor (192 aa), FASTA scores: opt: 297, E(): 4.3e-12, (37.45% identity in 187 aa overlap); BAB50853|MLR4117 from Rhizobium loti (Mesorhizobium loti) (205 aa), FASTA scores: opt: 280, E(): 5.5e-11, (31.45% identity in 194 aa overlap); BAB53760|MLL8133 from Rhizobium loti (Mesorhizobium loti) (194 aa), FASTA scores: opt: 270, E(): 2.3e-10, (34.05% identity in 185 aa overlap); etc. Also similar to other regulators from Mycobacterium tuberculosis e.g. P96839|Rv3557c|MTCY06G11.04c (200 aa), FASTA scores: opt: 154, E(): 0.0013, (38.8% identity in 80 aa overlap). Contains probable helix-turn-helix motif from aa 39 to 60 (Score 1251, +3.45 SD). Similar to the TetR/AcrR family of transcriptional regulators. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | DNA microarrays show increased expression in M. tuberculosis H37Rv in BALB/c mice compared to SCID mice, after 21 days of infection (See Talaat et al., 2004). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3541443 | 3542045 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3173c|Rv3173c
MPPVTRTTEPPRRGGRGARQRILKAAAELFYCEGINATGVELIANKASVSKRTLYQHFPSKSALVEEYLRGLRQAAGEADKMPKASNATPRERLLALFDRPNRGDGRMRGCPFHNAAVEAAGEMPGVERIVHSHKRDYIKGLARLAREAGAAHPRSLGNQLAVLFEGAAALSTSLDDAGPWAHARAAAEVLIDQATARPV
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
