Gene Rv3176c (lipS)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Biotransformation enzyme that acts on a variety of epoxides and arene oxides. Catalyzes the hydrolysis of arene and epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water [catalytic activity: an epoxide + H(2)O = a glycol]. |
| Product | Probable epoxide hydrolase MesT (epoxide hydratase) (arene-oxide hydratase) |
| Comments | Rv3176c, (MTV014.20c), len: 318 aa. Probable mesT, epoxide hydrolase, similar to others e.g. O15007|PEG1|MEST|Q92571|O14973 MEST protein (mesoderm specific transcript (mouse) homolog) (similar to alpha/beta hydrolase fold) from Homo sapiens (Human) (335 aa), FASTA scores: opt: 348, E(): 6e-15, (32.15% identity in 280 aa overlap); AAH06639|Q07646 MEST protein from Mus musculus (Mouse) (335 aa), FASTA scores: opt: 342, E(): 1.4e-14, (31.45% identity in 280 aa overlap); Q9I8E7|MEST epoxide hydrolase from Fugu rubripes (Japanese pufferfish) (Takifugu rubripes) (326 aa), FASTA scores: opt: 322, E(): 2.7e-13, (29.55% identity in 301 aa overlap); Q9PUC9|PEG1|MEST epoxide hydrolase from Brachydanio rerio (Zebrafish) (Zebra danio) (344 aa), FASTA scores: opt: 322, E(): 2.8e-13, (32.35% identity in 207 aa overlap); Q9HYH6|PA3429 probable epoxide hydrolase from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 258, E(): 3e-09, (29.85% identity in 288 aa overlap); O31243|ECHA epoxide hydrolase from Agrobacterium radiobacter (294 aa), FASTA scores: opt: 202, E(): 1.1e-05, (27.0% identity in 278 aa overlap); etc. Also similar to Q50599|Rv1834|MT1882|MTCY1A11.09c hypothetical 31.7 KDA protein from Mycobacterium tuberculosis (288 aa), FASTA scores: opt: 294, E(): 1.5e-11, (29.95% identity in 287 aa overlap). Equivalent to AAK47604 from Mycobacterium tuberculosis strain CDC1551 (339 aa) but shorter 21 aa. Similar to alpha/beta hydrolase fold. May belong to peptidase family S33. Note that previously known as lipS. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Virulence, detoxification, adaptation |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3544344 | 3545300 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3176c|mesT
MTHRASALISAQEWFSAGERVGYDAERPGINPRSPLRAFIRRAAGTGVTRTFLPGWPDGSYGWAKVEAFLSSRFHFPRIYLDYIGHGDSDKPRDYPYSTFERADLVEALWHAEGIAQTVVVAFDYSCIVSLELLARRIDRERAGNDQRTRITACLLANGGIFADGHTHAWYTTPLLTSPLGAAITPIGQRSWRMFAPFLRPVFSRGYPLSAAEMKELHDAISRRDGVRVLPATAGFVDEHREHAARWDLARIISALGDEVAFGVVGSAEDPFEGEQLRLARERLADSVEITELAGGHLTTAEQPDRLAEVIAALPERS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Deb C, Daniel J, Sirakova TD, Abomoelak B, Dubey VS and Kolattukudy PE [2006]. A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis. Product Transcriptome
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
