Gene Rv3179
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv3179, (MTV014.23), len: 429 aa. Conserved protein, highly similar to Q9KH61 putative ATP/GTP binding protein from Mycobacterium smegmatis (428 aa), FASTA scores: opt: 2466, E(): 1.5e-148, (89.7% identity in 428 aa overlap) (no article found on the NCBI web site (July 2001)); and to other hypothetical bacterial proteins e.g. O07781|Rv0597c|MTCY19H5.25 from M. tuberculosis (411 aa), FASTA scores: opt: 1031, E(): 8e-58, (41.5% identity in 417 aa overlap); BAB54715|MLR9349 from Rhizobium loti (Mesorhizobium loti) (435 aa), FASTA scores: opt: 365, E(): 1.1e-15, (31.75% identity in 416 aa overlap); etc. Equivalent to AAK47609 from Mycobacterium tuberculosis strain CDC1551 (454 aa) but shorter 25 aa. Contains PS00017 ATP/GTP-binding site motif A (P-loop). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3547618 | 3548907 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3179|Rv3179
VVHDEAGHELIERHMLEQLREVAEYTRVVLINGPRQAGKTTLLQQLHAELGGWLRSLDVDVERASARADPEGYIMSAPRPTFLDEVQCAGDPLILAIKTATDRDRRPRQFFLSGSTRFLTVPTLSESLAGRVAILDLWPLSVAERSGVRPEIIAQLFTEPQVVLGTEPAPVTRHEYLQLACAGGFPEVVQRPAGRARSRWFSDYLRTVTQRDVRELKRIEQTDRLPRFMRYLAAITAQELNVAEAARVIGVDAGTIRSDLALFETVYLVHRLPAWSRNLTAKIKKRSKIHVVDSGFAAWLRGQSADSLARPTAEGAGPIMETFVINELMKLRAATELEVDLYHFRDRDGREIDCILQTPDSRVVGVEVKASATVNVHDFRHLSFARDRLGDEFITGVLFYTGARALPFGDRLMALPINLLWNGQSVSSL
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
