Gene Rv3183
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Possible transcriptional regulatory protein |
| Comments | Rv3183, (MTV014.27), len: 109 aa. Possible transcriptional regulator, similar to others e.g. Q9S1D9|YPPCP1.08c from Yersinia pestis (99 aa), FASTA scores: opt: 119, E(): 0.47, (40.55% identity in 74 aa overlap); Q9X153|TM1330 from Thermotoga maritima (111 aa), FASTA scores: opt: 115, E(): 0.91, (40.35% identity in 57 aa overlap); P95258|Rv1956|MTCY09F9.08c (alias AAK46277 putative DNA-binding protein from strain CDC1551) (149 aa), FASTA scores: opt: 116, E(): 1, (42.25% identity in 71 aa overlap). Also similar to O53467|Rv2021c|MTV018.08c from Mycobacterium tuberculosis (101 aa), FASTA scores: opt: 214, E(): 5.8e-07, (43.0% identity in 107 aa overlap). Contains probable helix-turn-helix motif from aa 51 to 72 (Score 1803, +5.33 SD). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Regulatory proteins |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3550715 | 3551044 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3183|Rv3183
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTELGTLQAYVAALGGHLRIVAEFGENTVELTA
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
