Gene Rv3201c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Has both ATPase and helicase activities |
| Product | Probable ATP-dependent DNA helicase |
| Comments | Rv3201c, (MTV014.45c), len: 1101 aa. Probable ATP-dependent DNA helicase, similar to others e.g. Q9FCK4|2SC3B6.08 from Streptomyces coelicolor (1222 aa), FASTA scores: opt: 1209, E(): 5.4e-63, (38.45% identity in 1199 aa overlap); P71561|PCRA_MYCTU|CRA|IVRD|Rv0949|MT0976|MTCY10D7.25c from Mycobacterium tuberculosis (771 aa), FASTA scores: opt: 403, E(): 6.5e-16, (28.15% identity in 717 aa overlap); Q9FCK5|2SC3B6.07 from Streptomyces coelicolor (1159 aa), FASTA scores: opt: 349, E(): 1.3e-12, (29.2% identity in 1144 aa overlap); Q9L3M1|UVRD from Prochlorococcus sp. (512 aa; fragment), FASTA scores: opt: 290, E(): 2e-09, (27.95% identity in 479 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). |
| Functional category | Information pathways |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3573731 | 3577036 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3201c|Rv3201c
MTQTAAPARYSPAELACALGLFPPTAEQAAVIAAPPGPLVVIAGAGAGKTETMAARVVWLVANGYAEPGQVLGLTFTRKAAGQLLRRVRSRLARLAGIGLGCGDPAACAPVVSTYHAFAGSLLRDYGLLLPLEPDTRLLSETELWQLAFDVVSGYDGVLCTDKSPAAVTSIVVRLWGQLGEHLVDTRALRDTHVELERLVHALPAGRYQRDRGPSQWLLRMLATQTQRAELVPLLDALGERMHAGKVMDFAMQMASAARLAATSPQVGQDLRRRYRVVLLDEYQDTGHAQRVVLSSLFGGGVDDGLALTAVGDPIQSIYGWRGASATNLPRFTTDFPLSDGTPAPVLELLTSWRNPPQALRVANGISAEARRRSVAVRALRPRPDAPPGAVRCALLPDVQAEREWIADHLRMRYQRAEADGVKPPTAAVLVRRNADAAAIADTLRARGIPAEVVGLAGLLSIPEVAEVVAMLRLVADPTAGAAAMRVLTGPRWRLGARDLAALWRRALTLSGESPSTASPESIAMAASADADNPCLADAISDPGSAEGYSVAGYGRIGALAGELSALRGRLGHSLPDLVAEVRRVLGVDCEVRASAPVSGGWAGPEHLDAFADVVAGYAERASARSSEASVAGLLAYLDVAEVVENGLPPAELTVACDRVQVLTVHAAKGLEWQVVAVAHLSRGVFPSTVSRSSWLTDPAELPPLLRGDRASAGAHGIPVLDTSAVADRKQLSDKISEHRRLLDRRRVDEERRLLYVAVTRAEDTLLVSGHHWGPTGTKPRGPSEFLCELKDIIDRSAAAGDPCGVVEQWASAPAGDERNPLCDNAIEAVWPADPLAARRGDVERGAALVAAAMSADLPGSTTDIDHPPRPGDAPWSTDVDALLAERAHAARGAPARGLPNHLSVSSLVELVGDPVGARQRLMCRLPKRPDPHAWLGDAFHAWVQQFYGAELLFDLGDLPGAADREVGDPEELAALQRAFTASSWAARTPAAVEVPFEMPIGDTVVRGRIDAVFVDPDGGATVVDWKTGKPPHGPAAMRQAAVQLAVYRLAWAALRGCPTSSVRTAFYYVRSGITVVPDELPAPGELAMLLTDCAGRRSDT
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
