Gene Rv3206c (moeZ)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in molybdopterin metabolism (synthesis) |
| Product | Probable molybdenum cofactor biosynthesis protein MoeB1 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase) |
| Comments | Rv3206c, (MTCY07D11.20), len: 392 aa. Probable moeB1, molybdopterin cofactor biosynthesis protein, equivalent to Q9CCG8|MOEZ|ML0817 protein probably involved in molybdopterin biosynthesis from Mycobacterium leprae (395 aa), FASTA scores: opt: 2285, E(): 3.3e-130, (86.45% identity in 391 aa overlap.) Very similar to members of the HESA/MOEB/THIF family e.g. Q9FCL0|2SC3B6.02 putative sulfurylase from Streptomyces coelicolor (392 aa), FASTA scores: opt: 1776, E(): 1.4e-99, (65.3% identity in 395 aa overlap); Q9XC37|PDTORFF MOEB-like protein (putative sulfurylase) from Pseudomonas stutzeri (Pseudomonas perfectomarina) (391 aa), FASTA scores: opt: 1526, E(): 1.5e-84, (59.1% identity in 391 aa overlap); O54307|MPT|MOEB MPT-synthase sulfurylase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (391 aa), FASTA scores: opt: 1309, E(): 1.8e-71, (52.95% identity in 387 aa overlap); P74344|MOEB|SLL1536 molybdopterin biosynthesis MOEB protein from Synechocystis sp. strain PCC 6803 (392 aa), FASTA scores: opt: 1308, E(): 2e-71, (50.65% identity in 397 aa overlap); etc. Also highly similar to O05792|MOEB2|Rv3116|MTCY164.26 putative molybdenum cofactor biosynthesis protein from Mycobacterium tuberculosis (389 aa), FASTA scores: opt: 1440, E(): 2.3e-79, (57.25% identity in 386 aa overlap). Has hydrophobic segment from ~45-71. Belongs to the HesA/MoeB/ThiF FAMILY. Note that previously known as moeZ. Thought to be differentially expressed within host cells (see citation below). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Transcriptomics | mRNA identified by DNA microarray analysis and up-regulated at high temperatures (see Stewart et al., 2002). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3582532 | 3583710 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3206c|moeB1
VSTSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDFDVVDESNLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPYVWGSIYRFEGQASVFWEDAPDGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLLVYDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDSGRKLALIDVRDPVEWDIVHIDGAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEALAAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY
Bibliography
- Triccas JA et al. [1999]. Use of fluorescence induction and sucrose counterselection to identify Mycobacterium tuberculosis genes expressed within host cells. Regulation
- Stewart GR et al. [2002]. Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays. Transcriptome Mutant Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Burns KE et al. [2005]. Reconstitution of a new cysteine biosynthetic pathway in Mycobacterium tuberculosis. Function Product
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
