Gene Rv3211
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Has a helix-destabilizing activity |
| Product | Probable ATP-dependent RNA helicase RhlE |
| Comments | Rv3211, (MTCY07D11.15c), len: 527 aa. Probable rhlE, ATP-dependent RNA helicase, equivalent (but shorter 22 aa) to Q9CCH3|RHLE|ML0811 putative ATP-dependent RNA helicase from Mycobacterium leprae (544 aa), FASTA scores: opt: 2497, E(): 8.7e-131, (74.75% identity in 531 aa overlap). Also highly similar to other RNA helicases e.g. Q9FBJ2|SCP8.29c from Streptomyces coelicolor (879 aa), FASTA scores: opt: 1458, E(): 3.6e-73, (52.5% identity in 522 aa overlap); Q9DF36 from Xenopus laevis (African clawed frog) (800 aa), FASTA scores: opt: 792, E(): 2.3e-36, (37.15% identity in 385 aa overlap); Q99Z38|dead|SPY1415 from Streptococcus pyogenes (759 aa), FASTA scores: opt: 779, E(): 1.1e-35, (37.1% identity in 380 aa overlap); P33906|dead|CSDA from Klebsiella pneumoniae (642 aa), FASTA scores: opt: 768, E(): 4e-35, (43.4% identity in 387 aa overlap); etc. Contains ATP/GTP-binding site motif A (PS00017) and dead-box subfamily ATP-dependent helicases signature (PS00039). Similar to dead/DEAH box helicase family and similar to helicase C-terminal domain. |
| Functional category | Information pathways |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3587798 | 3589381 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3211|rhlE
MTAVKHTTESTFAKLGVRDEIVRALGEEGIKRPFAIQELTLPLALDGEDVIGQARTGMGKTFAFGVPLLQRITSGDGTRPLTGAPRALVVVPTRELCLQVTDDLATAGKYLTAGPDTDDAAAVRRRLSVVSIYGGRPYEPQIEALRAGADVVVGTPGRLLDLCQQGHLQLGGLSVLVLDEADEMLDLGFLPDIERILRQIPADRQSMLFSATMPDPIITLARTFMVRPTHIRAEAPHSSAVHDATEQFVYRAHALDKVELVSRVLQARDRGATMIFTRTKRTAQKVADELTERGFAVGAVHGDLGQLAREKALKAFRTGGIDVLVATDVAARGIDIDDVTHVINYQCPEDEKMYVHRIGRTGRAGRTGVAVTLVDWDELPRWSMIDQALGLGSPDPAETYSNSPHLYAELAIPATAGGTVGPARKSQGRRRDTDCDGQKTAQHARNTPRRRRTRGGKPVTGHPGTNPISSPIVGGDATSEPGSGTASDSGSDVVSGSRSGNGEAARRRRRRRRRPTHAQDGFAARAN
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Rodrigue S et al. [2007]. Identification of mycobacterial sigma factor binding sites by chromatin immunoprecipitation assays. Regulon
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
