Gene Rv3228
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3228, (MTCY20B11.03), len: 330 aa. Conserved hypothetical protein, equivalent to Q9CCI4|ML0791 hypothetical protein from Mycobacterium leprae (327 aa), FASTA scores: opt: 1828, E(): 1e-98, (84.0% identity in 331 aa overlap). Also similar to several hypothetical bacterial proteins e.g. Q9K4A8|SC7E4.08c from Streptomyces coelicolor (337 aa), FASTA scores: opt: 1051, E(): 1e-53, (52.65% identity in 338 aa overlap); Q9HUL3|PA4952 from Pseudomonas aeruginosa (339 aa), FASTA scores: opt: 392 ,E(): 1.4e-15, (34.85% identity in 281 aa overlap); Q9PFV1|XF0556 from Xylella fastidiosa (341 aa), FASTA scores: opt: 367, E(): 4e-14, (36.85% identity in 247 aa overlap); P45339|YJEQ_HAEIN|HI1714 from Haemophilus influenzae (346 aa), FASTA scores: opt: 355, E(): 2e-13, (31.65% identity in 281 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3604726 | 3605718 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3228|Rv3228
LRPGDYDESDVKVRSGRSSRPRTKTRPEHADAEAAMVVSVDRGRWGCVLGGRPDRRITAMRARELGRTPIVVGDDVDVVGDLSGRPDTLARIVRRAPRRTVLRRTADDTDPTERVVVANADQLLIVVALADPPPRTGLVDRALIAAYAGGLTPILCLTKTDLAPAEPFGKQFADLELTVTAAGVDDPLLAVADLLAGKITVLLGHSGVGKSTLVNRLVPEADRAVGEVTEIGRGRHTSTRSVALPLGDTLSGSGWVIDTPGIRSFGLAHIQPDNVLLAFSDLAEATRECPRGCGHMGPPADPECALDTLSGPAARRAAAARRLLAVLSQT
Bibliography
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
