Gene Rv3234c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | May be involved in synthesis of triacylglycerol |
| Product | Putative triacylglycerol synthase (diacylglycerol acyltransferase) Tgs3 |
| Comments | Rv3234c, (MTCY20B11.09c), len: 271 aa. Putative tgs3, triacylglycerol synthase (See Daniel et al., 2004), similar to C-terminus of Mycobacterium tuberculosis hypothetical proteins e.g. P71694|Rv1425|MTCY21B4.43|MTCY493.29c (459 aa), FASTA scores: opt: 498, E(): 5.2e-24, (36.8% identity in 261 aa overlap); MTCY03A2.28; MTCY31.23; MTCY493_29; MTCY28_26; MTV013_8; MTY13E12_33; etc. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 309, E(): 4.3e-12, (33.35% identity in 189 aa overlap). |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3610374 | 3611189 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3234c|tgs3
VVTRLSASDASFYQLENTATPMYVGLLLILRRPRAGLSYEALLETVEQRLPQIPRYRQKVQEVKLGLARPVWIDDRDFDITYHVRRSALPSPGSDEQLHELIARLAARPLDKSRPLWEMYLVEGLEKNRIALYTKSHQALINGVTALAIGHVIADRTRRPPAFPEDIWVPERDPGTTRLLLRAVGDWLVRPGAQLQAVGSAVAGLVTNSGQLVETGRKVLDIARTVARGTAPSSPLNATVSRNRRFTVARASLDDYRTVRARYDCDSTTWC
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Daniel J, Deb C, Dubey VS, Sirakova TD, Abomoelak B, Morbidoni HR and Kolattukudy PE [2004]. Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture. Function Product
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
