Gene Rv3239c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown, but seems involved in efflux system (probably sugar or drug transport). |
| Product | Probable conserved transmembrane transport protein |
| Comments | Rv3239c, (MTCY20B11.14c), len: 1048 aa. Probable conserved transmembrane protein, organised in two domains. Domain comprising first ~500 aa residues is similar to various antibiotic resistance and efflux proteins and contains sugar transport proteins signature 1 (PS00216); e.g. Q9RL22|SC5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 905, E(): 3.1e-41, (36.95% identity in 482 aa overlap); and O68912|FRNF putative antibiotic antiporter from Streptomyces roseofulvus (517 aa), FASTA scores: opt: 866, E(): 4.1e-39, (37.1% identity in 512 aa overlap). Second part, corresponding to last 550 aa residues, is very similar to Q50733|Rv2565|MTCY9C4.03c hypothetical 62.1 kDa protein from Mycobacterium tuberculosis (583 aa), FASTA scores: E(): 2.1e-28, (36.5% identity in 572 aa overlap). Also equivalent to Rv3728|MTV025.076 putative two-domain membrane protein (similar to sugar transporter family) from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 4328, E(): 0, (64.15% identity in 1046 aa overlap); and similar to other Mycobacterium tuberculosis proteins: MTCY3G12.01, E(): 6.3e-32; MTCY98.02c, E(): 6.3e-32; MTCY9C4.03c, E(): 1.5e-26; MTCY369.27c, E(): 2.5e-26. Equivalent to AAK47679 Drug transporter from Mycobacterium tuberculosis strain CDC1551 (1065 aa) but shorter 20 aa. Contains cyclic nucleotide-binding domain signature 2 (PS00889). Probably member of major facilitator superfamily (MFS). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 and 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3614457 | 3617603 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3239c|Rv3239c
MHISLHGGKGFANLTRRRRPSSASVLLVAGFGAFLAFLDSTIVNIAFPDIQRSFPSYDIGSLSWILNGYNIVFAAFMVAAGRLADLLGRRRTFLSGVLVFTIASGLCAVAGSVEQLVAFRVLQGIGAAILVPASLALVVEGFDAARRAHAIGLWGAAAAIAAGLGPPIGGLLVEWAGWRWVLLVNVPLGIVAAIATKRMLVESRASGRRRMPDLRGALLLAVTLGLVTLGLVKGPDWGWLSVATVGSFLASVLTSVGFVHSSRSHPAPLVEPALLRSRSFVAGNLLTLVAAAGFYCYGLTHVLYLNYVWHYSLLKAGFAIAPAAVVAAVVAAALGRVAGRHGHRVIVLVGALVWAGSLVWYLQRVGSEPDFLRVWLPGQLLQGIGVGATLPVLSSAALAEVAKGGSYATSSAVVSTTRQLGAVLGVAVMVILIGKPEHGTAEEALRRGWAMAAICFIAVAVAAAVLGRTNRNPVQMPAPEPAIAPRLEPPIPQPAAAPIEHWAAGDADPLGNLPLFAGLDAATLAQLGEHVEDVELEAGCYLFHEGDPSDSLYVIRTGRVQVLQDSIVLKELGRGEVLGELGLLIDAPRSATVRALRDTKLVRLTKAQFDEIADHGALAALVKVLATRLREAPPPATDSTSPEVVVSVIGVSGDAPVPAVAAGLLTALSARLRAVDPGRVDRDGLDRAERVADKVVLHAAVEDAGWRDFCLRVADRIVLVAGDPNPQAARLPARARGADLVLAGPAASREHRRQWEELITPRSVHVVHYRRILENVRPLAARIAGRSIGLVLGGGGARGFAHLGVLDELERVGVTIDRFAGTSMGAVIAVFGACGMDAATADAYAYEYFIRHNPLSDYAFPVRGLVRGRRTLTLLEAAFGDRLVEELPKEFRCVSVDLLARRPVVHRRGRLVDVIGCSLRLPGIYPPQVYNGRLHVDGGVLDNLPVSTRASPDGPLIAVSIGLGGGGPGSARQDGSPKVPGIGDTLMRTMTIGSQRGADAALSLAQVVIRPDTGAVGLLEFHQIDAAREAGRVAAREAMPHIMALLNR
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
