Gene Rv3257c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | This enzyme converses D-mannose 1-phosphate in D-mannose 6-phosphate [catalytic activity: D-mannose 1-phosphate = D-mannose 6-phosphate]. |
| Product | Probable phosphomannomutase PmmA (PMM) (phosphomannose mutase) |
| Comments | Rv3257c, (MTV015.02c), len: 465 aa. Probable pmmA, phosphomannomutase, equivalent to Q9CCJ7|PMMA|ML0763 phosphomannomutase from Mycobacterium leprae (468 aa), FASTA scores: opt: 2533, E(): 2e-145, (83.1% identity in 468 aa overlap). Also similar to many e.g. Q9KZL6|MANB from Streptomyces coelicolor (454 aa), FASTA scores: opt: 1820, E(): 2e-102, (63.2% identity in 459 aa overlap); Q9PGN8|XF0260 from Xylella fastidiosa (500 aa), FASTA scores: opt: 1085, E(): 4.7e-58, (40.7% identity in 462 aa overlap); Q9EY19|MANB from Salmonella enterica subsp. arizonae (456 aa), FASTA scores: opt: 988, E(): 3.1e-52, (38.65% identity in 445 aa overlap); etc. Belongs to the phosphohexose mutases family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3637312 | 3638709 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3257c|pmmA
MSWPAAAVDRVIKAYDVRGLVGEEIDESLVTDLGAAFARLMRTEDARPVVIGHDMRDSSPSLADAFAAGVTGQGLDVVRVGLASTDQLYFASGLLDCPGAMFTASHNPAAYNGIKMCRAAAKPVGADTGLTAIRDDLIAGVARYDGTPGTIADQDVLVDYGAFLRSLVDTSGLRPLRVAVDAGNGMAGHTAPAVLGVIDSITLLPSYFELDGSFPNHEANPLDPANLVDLQAYVRDTGADIGLAFDGDADRCFVVDERGQPVSPSTVTALVAARELNREIGATIIHNVITSRAVPELVAERGGTPLRSRVGHSYIKALMAETGAIFGGEHSAHYYFRDFWGADSGMLAALHVLAALGEQSRPLSELTADYQRYESSGEINFTVVDSSACVEAVLKSFGNRIVSIDHLDGVTVDLGDDSWFNLRSSNTEPLLRLNVEGRSVGDVDAVVRQVSAEIAAQSAHAKAGP
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
