Gene Rv3261
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Required for coenzyme F420 production: involved in the conversion of FO into F420. |
| Product | Probable F420 biosynthesis protein FbiA |
| Comments | Rv3261, (MTCY71.01), len: 331 aa. Probable fbiA, F420 biosynthesis protein, equivalent to FBIA F420 biosynthesis protein fbiA from Mycobacterium bovis BCG (see citations below). Also equivalent, but shorter 46 aa, to Q9CCK1|ML0759 hypothetical protein from Mycobacterium leprae (379 aa), FASTA scores: opt: 1855, E(): 3.9e-110, (79.3% identity in 333 aa overlap). Also similar to others e.g. Q9KZK9|SCE34.17 hypothetical 33.6 KDA protein from Streptomyces coelicolor (319 aa), FASTA scores: opt: 1151, E(): 1.2e-65, (55.1% identity in 332 aa overlap); O29345|AF0917 conserved hypothetical protein from Archaeoglobus fulgidus (296 aa), FASTA scores: opt: 469, E(): 1.7e-22, (31.15% identity in 302 aa overlap); Q58653|MJ1256 hypothetical protein from Methanococcus jannaschii (311 aa), FASTA scores: opt: 436, E(): 2.2e-20, (27.35% identity in 274 aa overlap); etc. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3640543 | 3641538 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3261|fbiA
VKVTVLAGGVGGARFLLGVQQLLGLGQFAANSAHSDADHQLSAVVNVGDDAWIHGLRVCPDLDTCMYTLGGGVDPQRGWGQRDETWHAMQELVRYGVQPDWFELGDRDLATHLVRTQMLQAGYPLSQITEALCDRWQPGARLLPATDDRCETHVVITDPVDESRKAIHFQEWWVRYRAQVPTHSFAFVGAEKSSAATEAIAALADADIIMLAPSNPVVSIGAILAVPGIRAALREATAPIVGYSPIIGEKPLRGMADTCLSVIGVDSTAAAVGRHYGARCATGILDCWLVHDGDHAEIDGVTVRSVPLLMTDPNATAEMVRAGCDLAGVVA
Bibliography
- Choi KP et al. [2001]. Use of transposon Tn5367 mutagenesis and a nitroimidazopyran-based selection system to demonstrate a requirement for fbiA and fbiB in coenzyme F(420) biosynthesis by Mycobacterium bovis BCG. Homolog Mutant
- Choi KP et al. [2002]. Demonstration that fbiC is required by Mycobacterium bovis BCG for coenzyme F(420) and FO biosynthesis. Homolog Secondary Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
