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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionPossibly a sulfotransferase involved in the formation of thiosulfate [catalytic activity: thiosulfate + cyanide = sulfite + thiocyanate].
ProductProbable thiosulfate sulfurtransferase SseA (rhodanese) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase)
CommentsRv3283, (MTCY71.23), len: 297 aa. Probable sseA, thiosulfate sulfurtransferase, equivalent P46700|THT2_MYCLE|SSEA|ML0728|B1308_C1_127 putative thiosulfate sulfurtransferase SSEA from Mycobacterium leprae (296 aa), FASTA scores: opt: 1742, E(): 5.5e-108, (83.45% identity in 296 aa overlap). Also highly similar to others e.g. Q9RXT9|DR0217 from Deinococcus radiodurans (286 aa), FASTA scores: opt: 1057, E(): 1.2e-62, (53.86% identity in 273 aa overlap); P16385|THTR_SACER|CYSA from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa), FASTA scores: opt: 1006, E(): 2.7e-59, (51.25% identity in 277 aa overlap); P71121|THTR_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (225 aa), FASTA scores: opt: 897, E(): 3.6e-52, (59.05% identity in 215 aa overlap); etc. Also highly similar to O05793|CYSA1|CYSA|Rv3117|MT3199|MTCY164.27|CYSA2|RV0815c|MT0837|MTV043. 07c|THTR_MYCTU putative thiosulfate sulfurtransferase from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 955, E(): 6.3e-56, (50.2% identity in 271 aa overlap); and Q50036|THTR_MYCLE|CYSA|CYSA3|ML2198 putative thiosulfate sulfurtransferase from Mycobacterium leprae (277 aa), FASTA scores: opt: 931, E(): 2.5e-54, (48.9% identity in 276 aa overlap). Shows some similarity to MTCY339.19c (30.3% identity in 254 aa overlap). Contains PS00683 Rhodanese C-terminal signature. Belongs to the rhodanese family. Thought to be differentially expressed within host cells (see Triccas et al., 1999).
Functional categoryIntermediary metabolism and respiration
ProteomicsThe product of this CDS corresponds to a spot identified in cytosol by proteomics at the Statens Serum Institute (Denmark) (see proteomics citations). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cytosol and cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Slow growth mutant by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al.,2003). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS36649283665821+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3283|sseA
VPLPADPSPTLSAYAHPERLVTADWLSAHMGAPGLAIVESDEDVLLYDVGHIPGAVKIDWHTDLNDPRVRDYINGEQFAELMDRKGIARDDTVVIYGDKSNWWAAYALWVFTLFGHADVRLLNGGRDLWLAERRETTLDVPTKTCTGYPVVQRNDAPIRAFRDDVLAILGAQPLIDVRSPEEYTGKRTHMPDYPEEGALRAGHIPTAVHIPWGKAADESGRFRSREELERLYDFINPDDQTVVYCRIGERSSHTWFVLTHLLGKADVRNYDGSWTEWGNAVRVPIVAGEEPGVVPVV
      
Bibliography