Gene Rv3293 (aldB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in L-alpha-aminoadipic acid (L-AAA) biosynthesis (in the second step; the first step is promoted by lat enzyme. |
| Product | Probable piperideine-6-carboxilic acid dehydrogenase Pcd (piperideine-6-carboxylate dehydrogenase) |
| Comments | Rv3293, (MTCY71.33), len: 494 aa. Probable pcd, piperideine-6-carboxylic acid dehydrogenase, highly similar to others e.g. O85725|PCD semialdehyde dehydrogenase from Streptomyces clavuligerus (512 aa), FASTA scores: opt: 2214, E(): 6.7e-121, (68.75% identity in 496 aa overlap) (see Alexander & Jensen 1998); Q9I4U7|PA1027 probable aldehyde dehydrogenase from Pseudomonas aeruginosa (529 aa), FASTA scores: opt: 1984, E(): 1.4e-107, (64.5% identity in 493 aa overlap); BAB49892|MLL2867 aldehyde dehydrogenase from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 1964, E(): 2e-106, (62.8% identity in 476 aa overlap); Q9A8Y1|CC1216 aldehyde dehydrogenase from Caulobacter crescentus (507 aa), FASTA scores: opt: 1909, E(): 3.1e-103, (59.95% identity in 497 aa overlap); O54199|PCD piperideine-6-carboxilic acid dehydrogenase from Streptomyces clavuligerus (496 aa), FASTA scores: opt: 1748, E(): 6.4e-94, (60.6% identity in 467 aa overlap); and Q9F1U8|PCD piperideine-6-carboxylate dehydrogenase from 'Flavobacterium' lutescens (510 aa), FASTA scores: opt: 1656, E(): 1.4e-88, (54.05% identity in 481 aa overlap) (see Fujii et al., 2000); etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Note that ORF Rv3290c seems to encoded the putative lat enzyme. Note that previously known as aldB. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 96h of starvation (see Betts et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3673602 | 3675086 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3293|pcd
VLEACQAIGVTAALGEPGEHSLPASTPITGDVLFSIAPTTPEQADHAIAAAAATFTAWRSTPAPVRGALVARLGELLTAHQQDLATLVTVEVGKITAEARGEVQEMIDVCQFSVGLSRQLYGRTIASERAGHRLLETWHPLGVVGVITAFNFPVAVWAWNTAVALVCGDTVVWKPSELTPLTALACQALLSRAAADVGAPAAVGGLLLGGAERGAQLVDDPRVALLSATGSVRMGQQVGPRVARRFGRVLLELGGNNAAIVAPSADLELAVRGIVFAAAGTAGQRCTSLRRLIVHRSVADDVVARVVGAYRQLAIGDPSAPDTLVGPLIHEAAYRDMVAALERARTDGGEVIGGDRREVGSPGAYYVAPAVVRMPSQTAIVATETFAPILYVLTYDDLDEAIALNNAVPQGLSSSIFTTDLREAEHFLDQSDCGIANVNIGTSGAEIGGAFGGEKQTGGGRESGSDAWKAYMRRATNTVNYSSELPLAQGVKFG
Bibliography
- Pérez-Llarena FJ et al. [1998]. The pcd gene encoding piperideine-6-carboxylate dehydrogenase involved in biosynthesis of alpha-aminoadipic acid is located in the cephamycin cluster of Streptomyces clavuligerus. Homolog Sequence Product
- Alexander DC et al. [1998]. Investigation of the Streptomyces clavuligerus cephamycin C gene cluster and its regulation by the CcaR protein. Homolog Sequence Mutant
- Fujii T et al. [2000]. Characterization of L-lysine 6-aminotransferase and its structural gene from Flavobacterium lutescens IFO3084. Homolog Sequence Product
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
