Gene Rv3305c (amiA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; certainly hydrolyses L-amino acid. |
| Product | Possible N-acyl-L-amino acid amidohydrolase AmiA1 (N-acyl-L-amino acid aminohydrolase) |
| Comments | Rv3305c, (MTV016.04c), len: 389 aa. Possible amiA1, N-acyl-L-amino acid amidohydrolase (or peptidase), similar to many proteins e.g. Q9AK43|2SCK8.09 putative peptidase from Streptomyces coelicolor (410 aa), FASTA scores: opt: 1015, E(): 3.9e-54, (50.8% identity in 374 aa overlap); Q9UZ30|PAB0873 amino acid amidohydrolase from Pyrococcus abyssi (383 aa), FASTA scores: opt: 823, E(): 1.6e-42, (38.2% identity in 369 aa overlap); O58453|PH0722 long hypothetical amino acid amidohydrolase from Pyrococcus horikoshii (388 aa), FASTA scores: opt: 815, E(): 4.8e-42, (38.75% identity in 369 aa overlap); O34980|YTNL_BACSU hypothetical 45.2 KDA protein from B. subtilis (416 aa), FASTA scores: opt: 805, E(): 2.1e-41, (37.85% identity in 367 aa overlap); Q9KCF8|BH1613 N-acyl-L-amino acid amidohydrolase from Bacillus halodurans (404 aa), FASTA scores: opt: 795, E(): 8.1e-41, (37.7% identity in 382 aa overlap); BAB50445|MLR3583 hypothetical hippurate hydrolase from Rhizobium loti (Mesorhizobium loti) (387 aa), FASTA scores: opt: 761, E(): 8.9e-39, (37.65% identity in 385 aa overlap); Q9RXH4|DR0339 putative N-acyl-L-amino acid amidohydrolase from Deinococcus radiodurans (392 aa), FASTA scores: opt: 745, E(): 8.4e-38, (36.15% identity in 379 aa overlap); etc. Contains PS00639 Eukaryotic thiol (cysteine) proteases histidine active site. Note that previously known as amiA. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3691639 | 3692808 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3305c|amiA1
MSLADAAESWLAAHHDDLVGWRRHIHRYPELGRQEYATTQFVAERLADAGLNPKVLPGGTGLTCDFGPQHQPRIALRADMDALPMAERTGAPYASTMPNVAHACGHDAHTAILLGAALALASVPELPVGVRLIFQAAEELMPGGAIDAIAAGALAGVSRIFALHCDPRLEVGKVAVRQGPITSAADSIEITLYSPGGHTSRPHLTADLVYGLGTLVTGLPGVLSRRIDPRNSTVLVWGAVNAGMAANAIPQTGVLSGTVRTASRQTWVDLEELVRQAISALLLPLAIEHTLQYRRGVPPVVNEEISTRILAHAIEAIGPGVLADTRQSGGGEDFSWYLEEVPGAMARLGVWSGDGLQLDLHQPTFDIDERALAIGLRVMVNIIEQAAAH
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
