Gene Rv3308
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Converts D-mannose 1-phosphate to D-mannose 6-phosphate. |
| Product | Probable phosphomannomutase PmmB (phosphomannose mutase) |
| Comments | Rv3308, (MTV016.07), len: 534 aa. Probable pmmB, phosphomannomutase, equivalent to Q9CCL7|PMMB|ML0706 putative phospho-sugar mutase from Mycobacterium leprae (538 aa), FASTA scores: opt: 2681, E(): 1.4e-150, (76.95% identity in 538 aa overlap). Also similar to others e.g. Q9AD82|SCK13.08c from Streptomyces coelicolor (549 aa), FASTA scores: opt: 1378, E(): 8.9e-74, (46.7% identity in 529 aa overlap); Q9ZHL4|PMM (fragment so no homology at N-terminus for this one) from Haemophilus ducreyi (443 aa), FASTA scores: opt: 935, E(): 9.6e-48, (39.4% identity in 449 aa overlap); P18159|YHXB_BACSU from Bacillus subtilis (565 aa), FASTA scores: opt: 776, E(): 2.7e-38, (31.7% identity in 574 aa overlap); etc. Contains PS00710 Phosphoglucomutase and phosphomannomutase phosphoserine signature. Belongs to the phosphohexose mutases family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3694864 | 3696468 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3308|pmmB
VTPENWIAHDPDPQTAAELAACGPDELKARFSRPLAFGTAGLRGHLRGGPDAMNLAVVLRATWAVARVLTDRGLAGSPVIVGRDARHGSPAFAAAAAEVLAAAGFSVLLLPDPAPTPVVAFAVRHTGAAAGIQITASHNPATDNGYKVYVDGGLQLLAPTDRQIEAAMATAPPADQIARKTVNPSENRASDLIDRYIQRAAGVRRCAGSVRVALTPLHGVGGAMAVETLRRAGFTEVHTVATQFAPNPDFPTVTLPNPEEPGATDALLTLATDVDADVAIALDPDADRCAVGIPTVSGWRMLSGDETGWLLGDYILSQTDDRASPPETRVVASTVVSSRMLAAIAAHHAAVHVETLTGFKWLARADANLPGTLVYAYEEAIGHCVDPTAVRDKDGISAAVLVCDLVAALKGQGRSVTDALDELARCYGVHEVAALSRPVSGAVETTDLMRRLREDPPRRLAGFPATVTDIGDTLILTGGDDNMLVRVAVRPSGTEPKLKCYLEIRCAVTGDLPAARQLVRARIDELSASVRRWW
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
