Gene Rv3370c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The alpha chain is the DNA polymerase. [catalytic activity: N deoxynucleoside triphosphate = N pyrophosphate + DNA(N)]. |
| Product | Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase) |
| Comments | Rv3370c, (MTV004.28c), len: 1079 aa. Probable dnaE2, DNA polymerase III, alpha chain (see citations below), similar to many e.g. BAB51086|MLR4428 from Rhizobium loti (Mesorhizobium loti) (1118 aa), FASTA scores: opt: 1103, E(): 8.9e-59, (37.65% identity in 1075 aa overlap); Q9S291|SCI11.28c from Streptomyces coelicolor (1185 aa), FASTA scores: opt: 937, E(): 1e-48, (33.4% identity in 1090 aa overlap); O67125|DP3A_AQUAE|DNAE|AQ_1008 from Aquifex aeolicus (1161 aa), FASTA scores: opt: 895, E(): 3.4e-46, (29.9% identity in 1071 aa overlap); O51526|DP3A_BORBU from Borrelia burgdorferi (Lyme disease spirochete) (1147 aa), FASTA scores: opt: 835, E(): 1.4e-42, (30.05% identity in 888 aa overlap); etc. Equivalent to AAK47817 from Mycobacterium tuberculosis strain CDC1551 (1098 aa) but shorter 19 aa. Also similar to Mycobacterium tuberculosis DP3A_MYCTU|MTCY48.18c|dnaE1 (29.6% identity in 1110 aa overlap). Belongs to DNA polymerase type-C family, DNAE subfamily. |
| Functional category | Information pathways |
| Proteomics | Identified in the cell wall and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | mRNA identified by Microarray analysis (see Davis et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3781501 | 3784740 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3370c|dnaE2
MERVLNGKPRHAGVPAFDADGDVPRSRKRGAYQPPGRERVGSSVAYAELHAHSAYSFLDGASTPEELVEEAARLGLCALALTDHDGLYGAVRFAEAAAELDVRTVFGAELSLGATARTERPDPPGPHLLVLARGPEGYRRLSRQLAAAHLAGGEKGKPRYDFDALTEAAGGHWHILTGCRKGHVRQALSQGGPAAAQRALADLVDRFTPSRVSIELTHHGHPLDDERNAALAGLAPRFGVGIVATTGAHFADPSRGRLAMAMAAIRARRSLDSAAGWLAPLGGAHLRSGEEMARLFAWCPEAVTAAAELGERCAFGLQLIAPRLPPFDVPDGHTEDSWLRSLVMAGARERYGPPKSAPRAYSQIEHELKVIAQLRFPGYFLVVHDITRFCRDNDILCQGRGSAANSAVCYALGVTAVDPVANELLFERFLSPARDGPPDIDIDIESDQREKVIQYVYHKYGRDYAAQVANVITYRGRSAVRDMARALGFSPGQQDAWSKQVSHWTGQADDVDGIPEQVIDLATQIRNLPRHLGIHSGGMVICDRPIADVCPVEWARMANRSVLQWDKDDCAAIGLVKFDLLGLGMLSALHYAKDLVAEHKGIEVDLARLDLSEPAVYEMLARADSVGVFQVESRAQMATLPRLKPRVFYDLVVEVALIRPGPIQGGSVHPYIRRRNGVDPVIYEHPSMAPALRKTLGVPLFQEQLMQLAVDCAGFSAAEADQLRRAMGSKRSTERMRRLRGRFYDGMRALHGAPDEVIDRIYEKLEAFANFGFPESHALSFASLVFYSAWFKLHHPAAFCAALLRAQPMGFYSPQSLVADARRHGVAVHGPCVNASLAHATCENAGTEVRLGLGAVRYLGAELAEKLVAERTANGPFTSLPDLTSRVQLSVPQVEALATAGALGCFGMSRREALWAAGAAATGRPDRLPGVGSSSHIPALPGMSELELAAADVWATGVSPDSYPTQFLRADLDAMGVLPAERLGSVSDGDRVLIAGAVTHRQRPATAQGVTFINLEDETGMVNVLCTPGVWARHRKLAHTAPALLIRGQVQNASGAITVVAERMGRLTLAVGARSRDFR
Bibliography
- Mizrahi V et al. [1998]. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Secondary Function
- Boshoff HI et al. [2003]. DnaE2 polymerase contributes to in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis. Mutant Gene
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
