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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in osmoregulatory trehalose biosynthesis. Mycobacteria can produce trehalose from glucose 6-phosphate and UDP-glucose (the OtsA-OtsB pathway) from glycogen-like alpha(1-->4)-linked glucose polymers (the TreY-TreZ pathway) and from maltose (the TreS pathway) [catalytic activity: trehalose 6-phosphate + H(2)O = trehalose + orthophosphate].
ProductTrehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP)
CommentsRv3372, (MTV004.30), len: 391 aa. otsB2, trehalose-6-phosphate phosphatase, equivalent to Q49734|OTSB2|OTSP|B1620_F1_1|MLCL383.17c putative trehalose-phosphatase from Mycobacterium leprae (429 aa), FASTA scores: opt: 1675, E(): 2.4e-91, (67.05% identity in 425 aa overlap). Also weakly similar to several trehalose phosphatases e.g. Q9C8B3|F10O5.8 from Arabidopsis thaliana (Mouse-ear cress) (366 aa), FASTA scores: opt: 432, E(): 3.1e-18, (36.65% identity in 281 aa overlap); O27788|MTH1760 from Methanobacterium thermoautotrophicum (264 aa), FASTA scores: opt: 347, E(): 2.5e-13, (30.75% identity in 221 aa overlap); Q9FWQ2 from Oryza sativa (Rice) (382 aa), FASTA scores: opt: 338, E(): 1.1e-12, (32.5% identity in 320 aa overlap); etc. Also similar to part of Mycobacterium tuberculosis Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), FASTA scores: opt: 1192, E(): 1.6e-62, (56.65% identity in 339 aa overlap).
Functional categoryVirulence, detoxification, adaptation
ProteomicsIdentified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in the culture filtrate and whole cell lysates of M. tuberculosis H37Rv but not the membrane protein fraction (See de Souza et al., 2011).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS37863143787489+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3372|otsB2
VRKLGPVTIDPRRHDAVLFDTTLDATQELVRQLQEVGVGTGVFGSGLDVPIVAAGRLAVRPGRCVVVSAHSAGVTAARESGFALIIGVDRTGCRDALRRDGADTVVTDLSEVSVRTGDRRMSQLPDALQALGLADGLVARQPAVFFDFDGTLSDIVEDPDAAWLAPGALEALQKLAARCPIAVLSGRDLADVTQRVGLPGIWYAGSHGFELTAPDGTHHQNDAAAAAIPVLKQAAAELRQQLGPFPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRTAEQRHALRVTTGREVIELRPDVDWDKGKTLLWVLDHLPHSGSAPLVPIYLGDDITDEDAFDVVGPHGVPIVVRHTDDGDRATAALFALDSPARVAEFTDRLARQLREAPLRAT